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Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. 2017

Sakari S Karhula, and Mikko A Finnilä, and Mikko J Lammi, and Janne H Ylärinne, and Sami Kauppinen, and Lassi Rieppo, and Kenneth P H Pritzker, and Heikki J Nieminen, and Simo Saarakkala
Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland.

Contrast-enhanced micro-computed tomography (CEμCT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to investigate the dependence of PTA for the collagen content in bovine articular cartilage. Adjacent bovine articular cartilage samples were treated with chondroitinase ABC and collagenase to degrade the proteoglycan and the collagen constituents in articular cartilage, respectively. Enzymatically degraded samples were compared to the untreated samples using CEμCT and reference methods, such as Fourier-transform infrared imaging. Decrease in the X-ray attenuation of PTA in articular cartilage and collagen content was observed in cartilage depth of 0-13% and deeper in tissue after collagen degradation. Increase in the X-ray attenuation of PTA was observed in the cartilage depth of 13-39% after proteoglycan degradation. The X-ray attenuation of PTA-labelled articular cartilage in CEμCT is associated mainly with collagen content but the proteoglycans have a minor effect on the X-ray attenuation of the PTA-labelled articular cartilage. In conclusion, the PTA labeling provides a feasible CEμCT method for 3D characterization of articular cartilage.

UI MeSH Term Description Entries
D007485 Ioxaglic Acid A low-osmolar, ionic contrast medium used in various radiographic procedures. Ioxaglate,Ioxaglate Meglumine,Ioxaglate Sodium,Hexabrix,Ioxaglic Acid Monosodium Salt,Ioxaglic Acid, Calcium Salt (2:1),Methylglucamine Ioxaglate,P-286 (Contrast Media),P286 (Contrast Media),Ioxaglate, Methylglucamine,Meglumine, Ioxaglate
D010772 Phosphotungstic Acid Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed) Acid, Phosphotungstic
D011509 Proteoglycans Glycoproteins which have a very high polysaccharide content. Proteoglycan,Proteoglycan Type H
D002358 Cartilage, Articular A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact. Articular Cartilage,Articular Cartilages,Cartilages, Articular
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017364 Collagenases Enzymes that catalyze the degradation of collagen by acting on the peptide bonds. Collagen Peptidase,Collagen-Degrading Enzyme,Collagenase,Collagen Degrading Enzyme,Peptidase, Collagen
D055114 X-Ray Microtomography X-RAY COMPUTERIZED TOMOGRAPHY with resolution in the micrometer range. MicroCT,Microcomputed Tomography,X-Ray Micro-CAT Scans,X-Ray Micro-CT,X-Ray Micro-CT Scans,X-Ray Micro-Computed Tomography,X-Ray Microcomputed Tomography,X-ray MicroCT,Xray Micro-CT,Xray MicroCT,Micro-CAT Scan, X-Ray,Micro-CAT Scans, X-Ray,Micro-CT Scan, X-Ray,Micro-CT Scans, X-Ray,Micro-CT, X-Ray,Micro-CT, Xray,Micro-CTs, X-Ray,Micro-CTs, Xray,Micro-Computed Tomography, X-Ray,MicroCT, X-ray,MicroCT, Xray,MicroCTs,MicroCTs, X-ray,MicroCTs, Xray,Microcomputed Tomography, X-Ray,Microtomography, X-Ray,Scan, X-Ray Micro-CAT,Scan, X-Ray Micro-CT,Scans, X-Ray Micro-CAT,Scans, X-Ray Micro-CT,Tomography, Microcomputed,Tomography, X-Ray Micro-Computed,Tomography, X-Ray Microcomputed,X Ray Micro CAT Scans,X Ray Micro CT,X Ray Micro CT Scans,X Ray Micro Computed Tomography,X Ray Microcomputed Tomography,X Ray Microtomography,X ray MicroCT,X-Ray Micro-CAT Scan,X-Ray Micro-CT Scan,X-Ray Micro-CTs,X-ray MicroCTs,Xray Micro CT,Xray Micro-CTs,Xray MicroCTs
D019765 Chondroitin ABC Lyase An enzyme that catalyzes the eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups. (Enzyme Nomenclature, 1992) Chondroitinase ABC,Chondroitin-Sulfate-ABC Endolyase,Chondroitin Sulfate ABC Endolyase

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