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A specific receptor for human atrial natriuretic peptide (h-ANP) was identified in the human kidney using the radioligand binding assay. Samples were prepared from non-malignant renal tissues obtained at nephrectomy of patients with renal carcinoma. Binding studies using [125I]hANP were performed at 0 degree C for 20 minutes and terminated by a rapid filtration technique. Scatchard plot analysis revealed [125I]hANP bound to a single class of binding site (Kd = 0.4 +/- 0.2 nM) with a density of 16 +/- 4 fmol/mg protein in the renal cortex (n = 7). The binding was rapid and maximal binding was obtained within 20 minutes after the start of incubation. Radioligand displacement was observed in a dose dependent fashion when cold hANP was entered into the reaction mixture. However, unrelated agents, such as angiotensin II or 1-epinephrine, did not affect the binding. This is the first time characterization of the hANP receptor in the human kidney has been conducted using a Scatchard plot analysis.
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
April 1995,
European journal of endocrinology,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
January 1992,
Endocrinology,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
April 1994,
Journal of nuclear medicine : official publication, Society of Nuclear Medicine,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
July 1990,
The American journal of physiology,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
January 1989,
Journal of cardiovascular pharmacology,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
May 1987,
Clinical endocrinology,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
July 1987,
American journal of kidney diseases : the official journal of the National Kidney Foundation,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
March 1995,
Nihon rinsho. Japanese journal of clinical medicine,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
May 1986,
Journal of clinical pathology,
Y Ishikawa, and
S Umemura, and
G Yasuda, and
K Uchino, and
T Shindou, and
K Minamizawa, and
Y Toya, and
Y Kaneko
November 1989,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
