-transparent.png){kind=logo}
Biochemical studies in vertebrate olfactory tissue indicate that certain odorants stimulate adenylyl cyclase in a GTP-dependent manner. Additionally, immunochemical and toxin-labeling studies demonstrate the presence of several GTP-binding protein (G-protein) species in vertebrate olfactory epithelium. To identify the G-protein(s) responsible for olfactory signal transduction, we screened a rat olfactory cDNA library with an oligonucleotide probe and isolated 32 recombinant clones encoding five distinct types of G-protein alpha subunits. The majority of the clones encoded G alpha s, while the remaining clones encoded G alpha o, G alpha i1, G alpha i2, and a novel species, G alpha i3. Messenger RNA corresponding to each G alpha was detectable in all tissues examined; however, the levels for a given G alpha varied in a tissue-specific manner. In olfactory tissue, G alpha s was the most abundant of these messages and in combination with the biochemical studies suggests that G alpha s is the G-protein component of the olfactory signal transduction cascade.
D T Jones, and
R R Reed
August 1993,
FEBS letters,
D T Jones, and
R R Reed
October 2000,
Genes & genetic systems,
D T Jones, and
R R Reed
October 1994,
FEBS letters,
D T Jones, and
R R Reed
May 1993,
Proceedings of the National Academy of Sciences of the United States of America,
D T Jones, and
R R Reed
November 1990,
FEBS letters,
D T Jones, and
R R Reed
October 1997,
Biochimica et biophysica acta,
D T Jones, and
R R Reed
June 1991,
Biochemical and biophysical research communications,
D T Jones, and
R R Reed
April 1994,
Biochemical and biophysical research communications,
D T Jones, and
R R Reed
October 2002,
DNA sequence : the journal of DNA sequencing and mapping,
D T Jones, and
R R Reed
March 1987,
Proceedings of the National Academy of Sciences of the United States of America,
