-transparent.png){kind=logo}
Single-step procedure for the extraction and purification of leukotrienes B4, C4 and D4. 1987
Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
A rapid and simple method for the on-line concentration and high-performance liquid chromatographic (HPLC) purification of the leukotrienes in good yield from biological fluids is described. Readily available antisera are used in conjunction with this system to give a specific and sensitive assay for leukotrienes B4, C4 and D4 with sub-nanogram limits of detection. Tritium-labelled leukotrienes are used as internal standards, both to locate the leukotrienes post-HPLC and to accurately determine recoveries.
Related Publications
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
June 1982,
Journal of immunology (Baltimore, Md. : 1950),
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
January 1983,
Agents and actions. Supplements,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
May 1987,
The Journal of surgical research,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
September 1985,
Prostaglandins,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
January 1989,
Journal of applied physiology (Bethesda, Md. : 1985),
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
November 1983,
British journal of pharmacology,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
July 1985,
The British journal of dermatology,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
August 1983,
Prostaglandins, leukotrienes, and medicine,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
January 1989,
Advances in prostaglandin, thromboxane, and leukotriene research,
R Richmond, and
N C Turner, and
N Maltby, and
D Heavey, and
J Vial, and
C T Dollery, and
G W Taylor
November 1999,
Nihon rinsho. Japanese journal of clinical medicine,
