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A safe, effective and economical vaccine is required for the prevention of Japanese encephalitis (JE), a disease with high mortality and grave sequelae, which is prevalent in Japan and other countries in east, south-east and southern Asia. As the initial step to produce a second-generation vaccine, recombinant DNA technology was utilized to express the JE virus envelope glycoprotein V3 (E) gene in yeast cells.This report describes the construction of a yeast expression vector in which a cDNA clone covering the V3 gene was connected to the acid-phosphatase promoter of a yeast vector plasmid. Successful expression of the V3 gene was detected by ELISA and Western blotting using monoclonal antibodies against JE V3. Immunization of mice with the V3 antigen expressed in yeast produced limited but definite levels of anti-JE antibodies which could neutralize JE virus. The results are an encouraging step in the development of a practical second-generation JE vaccine.
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
December 1989,
Acta virologica,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
January 1983,
Journal of virology,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
January 1987,
Archives of virology,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
May 1990,
Acta virologica,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
March 2003,
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
September 1982,
Acta virologica,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
June 1996,
Virus research,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
July 2001,
Archives of virology,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
June 2012,
The Journal of general virology,
H Fujita, and
H Sumiyoshi, and
C Mori, and
S Manabe, and
M Takagi, and
I Yoshida, and
K Morita, and
I Fuke, and
K Fukai, and
A Igarashi
April 1994,
Releve epidemiologique hebdomadaire,
