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OBJECTIVE To isolate a novel endophytic bacterium from Panax ginseng that could have excellent properties in converting ginsenoside Rb1 to ginsenoside Rg3. RESULTS Based on a 16S rDNA gene sequence, the strain named GE 17-7 was identified as Burkholderia sp. This strain has shown the highest activity in converting ginsenoside Rb1 to 20(S)-ginsenoside Rg3. During the biotransformation of ginsenoside Rb1, the final metabolite was identified by nuclear magnetic resonance analysis and the transformation pathway of ginsenoside Rb1 was also identified by thin-layer chromatography and high performance liquid chromatography analysis in this study. CONCLUSIONS We have successfully isolated a β-glucosidase-producing endophytic bacterium GE 17-7 from P. ginseng. Ginsenoside Rg3 was produced by strain GE 17-7 from ginsenoside Rb1 via ginsenoside Rd. CONCLUSIONS This is the first report of the conversion of major ginsenoside Rb1 into minor ginsenoside Rg3 by fermentation with Burkholderia sp. endophytic bacteria in P. ginseng. These results suggest a new preparation method for ginsenoside Rg3 using strain GE 17-7 in the pharmaceutical industry.
Y Fu, and
Z-H Yin, and
C-Y Yin
February 2019,
Letters in applied microbiology,
Y Fu, and
Z-H Yin, and
C-Y Yin
April 2021,
Antonie van Leeuwenhoek,
Y Fu, and
Z-H Yin, and
C-Y Yin
January 2017,
Korean journal for food science of animal resources,
Y Fu, and
Z-H Yin, and
C-Y Yin
May 2017,
Molecules (Basel, Switzerland),
Y Fu, and
Z-H Yin, and
C-Y Yin
January 2008,
Phytochemistry,
Y Fu, and
Z-H Yin, and
C-Y Yin
April 2012,
Applied microbiology and biotechnology,
Y Fu, and
Z-H Yin, and
C-Y Yin
November 2003,
Archives of pharmacal research,
Y Fu, and
Z-H Yin, and
C-Y Yin
November 2018,
Microbiology resource announcements,
Y Fu, and
Z-H Yin, and
C-Y Yin
October 2010,
Bioresource technology,
Y Fu, and
Z-H Yin, and
C-Y Yin
August 2023,
Canadian journal of microbiology,
