-transparent.png){kind=logo}
In direct binding assays, purified lambdaind+ repressor displayed high affinity for nonoperator DNA containing single-strand gaps. Its affinity for this same DNA but completely double-stranded, nicked, or denatured was considerably lower. In contrast, purified lambdaind- repressor had 1/10th the affinity for the gapped DNA, a level comparable to that of purified lac repressor. In the presence of limiting amounts of ind+ repressor, nonoperator DNA containing gaps could be shown to compete effectively with lambda DNA for binding of repressor. A previous model of lambda induction [Sussman, R. & Ben-Zeev, H. (1975) Proc. Natl. Acad. Sci. USA 72, 1973--1976], based on the assumption that this phenomenon involves the binding of repressor to lesions in the host DNA, is reevaluated in the light of the data reported here.
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
April 1981,
Nature,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
April 1983,
The Journal of biological chemistry,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
April 2001,
Biochemistry. Biokhimiia,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
July 1975,
Journal of molecular biology,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
January 1980,
Methods in enzymology,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
April 1976,
The Journal of biological chemistry,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
September 2013,
Protein expression and purification,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
December 2000,
FEBS letters,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
January 1972,
Molecular & general genetics : MGG,
R Sussman, and
J Resnick, and
K Calame, and
J Baluch
May 1987,
Journal of molecular biology,
