BIOMAT Database Logo BIOMAT Database Logo

Interaction of the MvaI restriction enzyme with synthetic DNA fragments. 1987

C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
Department of Chemistry, Humboldt University, Berlin, GDR.

The cleavage of synthetic DNA duplexes by the restriction endonuclease MvaI has been studied. The main result of the cleavage experiments is that MvaI cleaves unmodified duplexes in two single strand scissions in separate events and that the two strands are cleaved at significantly different rates. One strand nicks within the recognition site do not affect the cleavage. Furthermore, neither a pyrophosphate internucleotide bond modification in one strand nor the absence of one phosphate group at the central dA-residue of the recognition site do inhibit the cleavage of the second strand.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D009838 Oligodeoxyribonucleotides A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties. Oligodeoxynucleotide,Oligodeoxyribonucleotide,Oligodeoxynucleotides
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D015252 Deoxyribonucleases, Type II Site-Specific Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4. DNA Restriction Enzymes, Type II,DNase, Site-Specific, Type II,Restriction Endonucleases, Type II,Type II Restriction Enzymes,DNase, Site Specific, Type II,Deoxyribonucleases, Type II, Site Specific,Deoxyribonucleases, Type II, Site-Specific,Site-Specific DNase, Type II,Type II Site Specific DNase,Type II Site Specific Deoxyribonucleases,Type II Site-Specific DNase,Type II Site-Specific Deoxyribonucleases,Deoxyribonucleases, Type II Site Specific,Site Specific DNase, Type II

Related Publications

C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-strand cleavages.
December 1985, Nucleic acids research,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
[Formation of two types of enzyme-substrate complexes during the interaction of EcoRII restriction enzyme with synthetic DNA-duplexes].
January 1992, Molekuliarnaia biologiia,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
[Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. X. Hydrolysis of substrates with structural abnormalities].
September 1987, Bioorganicheskaia khimiia,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
[Mechanism of cleavage of DNA-duplexes by restriction endonuclease MvaI].
January 1987, Doklady Akademii nauk SSSR,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
[Interaction of EcoRII restriction and modification enzyme with synthetic DNA fragments. IV. DNA duplexes with phosphoamide and pyrophosphate internucleotide bonds--the substrates for the study of single-strand breaks].
January 1986, Molekuliarnaia biologiia,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
[Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. Determination of the size of EcoRII binding site].
January 1990, Molekuliarnaia biologiia,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
Kinetic studies of MvaI DNA methyltransferase interaction with modified oligonucleotide duplexes.
June 1995, Biochemistry and molecular biology international,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
Two-dimensional electrophoretic separation of restriction enzyme fragments of DNA.
January 1979, Methods in enzymology,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
[Interaction of restriction and modification enzyme EcoRII with synthetic DNA fragments. VII. The study of complex-formation of endonuclease EcoRII with substrates containing natural and modified recognition sites].
January 1986, Molekuliarnaia biologiia,
C D Pein, and D Cech, and E S Gromova, and T S Orezkaya, and Z A Shabarova, and E A Kubareva
Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. VI. The binding and cleavage of substrates containing nucleotide analogs.
December 1985, Nucleic acids research,
Copied contents to your clipboard!