Biomaterial Database

Hydrogenosomal ATP:AMP phosphotransferase of Trichomonas vaginalis. 1987

P J Declerck, and M Müller

Rockefeller University, New York, NY 10021.

1. ATP:AMP phosphotransferase (adenylate kinase) is present in Trichomonas vaginalis, primarily with hydrogenosomal localization. 2. Adenylate kinase has been purified from hydrogenosome-enriched fractions by solubilization with Triton X-100 and KCl followed by affinity chromatography and gel filtration. 3. The enzyme has a Mr = 28,000, a broad pH optimum of pH 7-9, requirement for Mg2+ and specificity for adenine and deoxyadenine nucleotides. 4. The activity is competetively inhibited by P1,P5-di(adenosine-5') pentaphosphate (Ki 200 nM) and reversibly inactivated by 5,5'-dithiobis-(2-nitrobenzoate). 5. Catalytic properties of this enzyme are similar to those of enzymes from other organisms. Other properties indicate its uniqueness, however, since its molecular mass and Ki for P1,P5-di(adenosine-5'-)-pentaphosphate bring it closer to the mitochrondrial isoenzyme, while it shares a requirement for reduced thiol groups with the cytosolic isoenzyme.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009940	Organoids	An organization of cells into an organ-like structure. Organoids can be generated in culture, e.g., self-organized three-dimensional tissue structures derived from STEM CELLS (see MICROPHYSIOLOGICAL SYSTEMS). They are also found in certain NEOPLASMS.	Organoid
D010770	Phosphotransferases	A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.	Kinases,Phosphotransferase,Phosphotransferases, ATP,Transphosphorylase,Transphosphorylases,Kinase,ATP Phosphotransferases
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000263	Adenylate Kinase	An enzyme that catalyzes the phosphorylation of AMP to ADP in the presence of ATP or inorganic triphosphate. EC 2.7.4.3.	Myokinase,AMP Kinase,ATP-AMP Phosphotransferase,ATP-AMP Transphosphorylase,Adenylokinase,ATP AMP Phosphotransferase,ATP AMP Transphosphorylase,Kinase, AMP,Kinase, Adenylate,Phosphotransferase, ATP-AMP,Transphosphorylase, ATP-AMP
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013347	Subcellular Fractions	Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)	Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction
D014246	Trichomonas vaginalis	A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.	Trichomonas vaginali,vaginali, Trichomonas