The Tudor Staphylococcal Nuclease Protein of Entamoeba histolytica Participates in Transcription Regulation and Stress Response. 2017

Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN Ciudad de México, Mexico.

Entamoeba histolytica is the protozoa parasite responsible of human amoebiasis, disease that causes from 40,000 to 100,000 deaths annually worldwide. However, few are known about the expression regulation of molecules involved in its pathogenicity. Transcription of some virulence-related genes is positively controlled by the cis-regulatory element named URE1. Previously we identified the transcription factor that binds to URE1, which displayed a nuclear and cytoplasmic localization. This protein belongs to the Tudor Staphyococcal nuclease (TSN) family, which in other systems participates in virtually all pathways of gene expression, suggesting that this amoebic transcription factor (EhTSN; former EhURE1BP) could also play multiple functions in E. histolytica. The aim of this study was to identify the possible cellular events where EhTSN is involved. Here, we found that EhTSN in nucleus is located in euchromatin and close to, but not into, heterochromatin. We also showed the association of EhTSN with proteins involved in transcription and that the knockdown of EhTSN provokes a diminishing in the mRNA level of the EhRabB gene, which in its promoter region contains the URE1 motif, confirming that EhTSN participates in transcription regulation. In cytoplasm, this protein was found linked to the membrane of small vesicles and to plasma membrane. Through pull-down assays and mass spectrometry we identity thirty two candidate proteins to interact with EhTSN. These proteins participate in transcription, metabolism, signaling, and stress response, among other cellular processes. Interaction of EhTSN with some candidate proteins involved in metabolism, and signaling was validated by co-immunoprecipitation or co-localization. Finally we showed the co-localization of EhTSN and HSP70 in putative stress granules during heat shock and that the knockdown of EhTSN increases the cell death during heat shock treatment, reinforcing the hypothesis that EhTSN has a role during stress response. All data support the proposal that EhTSN is a multifunctional protein of E. histolytica.

UI MeSH Term Description Entries
D008836 Micrococcal Nuclease An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1. Staphylococcal Nuclease,TNase,Thermonuclease,Thermostable Nuclease,Nuclease, Micrococcal,Nuclease, Staphylococcal,Nuclease, Thermostable
D010829 Physiological Phenomena The functions and properties of living organisms, including both the physical and chemical factors and processes, supporting life in single- or multi-cell organisms from their origin through the progression of life. Physiological Processes,Physiological Concepts,Physiological Phenomenon,Physiological Process,Concept, Physiological,Concepts, Physiological,Phenomena, Physiological,Phenomenas, Physiological,Phenomenon, Physiological,Physiological Concept,Process, Physiological,Processes, Physiological
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D004748 Entamoeba histolytica A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded. Endamoeba histolytica,histolytica, Endamoeba
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014157 Transcription Factors Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. Transcription Factor,Factor, Transcription,Factors, Transcription

Related Publications

Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
November 2016, Cell death and differentiation,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
May 2012, The Journal of biological chemistry,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
June 2014, FEBS letters,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
January 2015, Plant signaling & behavior,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
April 2008, International journal for parasitology,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
March 2015, The Plant cell,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
June 2011, International journal for parasitology,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
July 2008, Biochemical and biophysical research communications,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
February 2013, The Journal of biological chemistry,
Javier Cázares-Apátiga, and Christian Medina-Gómez, and Bibiana Chávez-Munguía, and Mercedes Calixto-Gálvez, and Esther Orozco, and Carlos Vázquez-Calzada, and Aarón Martínez-Higuera, and Mario A Rodríguez
February 2015, International journal of oncology,
Copied contents to your clipboard!