Biomaterial Database

[A microtitration and neutralization test for infectious laryngotracheitis virus. Their reproducibility and the effect of various tests conditions]. 1988

F W Hilbink, and D J van Roozelaar

Centraal Diergeneeskundig Instituut, Lelystad.

The reproducibility of micro-assays for ILTV was assessed. The standard deviation of the virus assay in three successive determinations was 0.4, 0.4 and 0.3 log TCID50. The micro-neutralisation test produced standard deviations of 0.6, 0.8, 0.5, 1.2 and 1.1 log2 in various experiments. Preincubation for 24 hours at 39 degrees C, rather than for sixty minutes at 22 degrees C, which was suggested by Bitsch to enhance sensitivity, resulted in antibody titres which were approximately 1 log2 higher, though the specificity of the test declined markedly. The dose-response relationship between virus and antibody titre was curvilinear, the steepest part of the slope being in the lower range. Thermal inactivation and freezing and thawing resulted in a significant reduction of serum titres (P less than 0.01). The following method is suggested as a practical standardised procedure: freezing of sera prior to processing: omission of thermal inactivation and preincubation for sixty minutes at 22 degrees C.

UI	MeSH Term	Description	Entries
D007158	Immunologic Techniques	Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.	Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D009500	Neutralization Tests	The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).	Neutralization Test,Test, Neutralization,Tests, Neutralization
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D006564	Herpesviridae	A family of enveloped, linear, double-stranded DNA viruses infecting a wide variety of animals. Subfamilies, based on biological characteristics, include: ALPHAHERPESVIRINAE; BETAHERPESVIRINAE; and GAMMAHERPESVIRINAE.	Mouse Thymic Virus,Murid herpesvirus 3,Thymic Group Viruses,Herpesviruses,Mouse Thymic Viruses,Thymic Virus, Mouse,Thymic Viruses, Mouse
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001352	Herpesvirus 1, Gallid	The type species of the genus ILTOVIRUS found on every continent and affecting mainly chickens and occasionally pheasants.	Avian Infectious Laryngotracheitis Virus,Gallid Herpesvirus 1,Herpesvirus 1 (alpha), Gallid,Infectious Laryngotracheitis Virus, Avian
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor