Biomaterial Database

Aptasensors for Detection of Avian Influenza Virus H5N1. 2017

Yanbin Li, and Ronghui Wang

Department of Biological and Agricultural Engineering, University of Arkansas, 230 Engineering Hall, Fayetteville, AR, 72701, USA. yanbinli@uark.edu.

Aptamers are single-stranded RNA or DNA oligonucleotides which have specific three-dimensional (3D) structures for high affinity and specific recognition to their target. In diagnostic and detection assays, aptamers represent an alternative to antibodies as recognition agents and offer advantages, such as reduced cost, rapid and reproducible synthesis, controllable modification, and improved stability. Aptamers are favorably used in biosensors as sensitive and selective bio-receptors coupled with a variety of transducers such as optical, mass-sensitive, and electrochemical sensors, the so-called aptasensors. We report the development of several types of aptasensors for rapid and specific detection of avian influenza virus (AIV) H5N1. DNA aptamers with high affinity and specificity against AIV H5N1 were immobilized on the electrode surface and then incorporated into different transducers such as surface plasmon resonance (SPR), quartz crystal microbalance (QCM) and electrochemical transducer. The target viruses were captured by the immobilized aptamers resulting in a detectable signal. The fabrication of aptasensors, detection principles, and their applications for AIV H5N1 detection are addressed.

UI	MeSH Term	Description	Entries
D008839	Microelectrodes	Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed)	Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D009690	Nucleic Acid Conformation	The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.	DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015374	Biosensing Techniques	Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.	Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D044085	Microfluidics	The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.	Microfluidic
D052157	Aptamers, Nucleotide	Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.	DNA Aptamer,DNA Aptamers,RNA Aptamers,Rna Aptamer,Nucleotide Aptamers,Oligonucleotide Ligands, DNA,Oligonucleotide Ligands, RNA,Aptamer, DNA,Aptamer, Rna,Aptamers, DNA,Aptamers, RNA,DNA Oligonucleotide Ligands,RNA Oligonucleotide Ligands
D053124	Influenza A Virus, H5N1 Subtype	A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 5 and neuraminidase 1. The H5N1 subtype, frequently referred to as the bird flu virus, is endemic in wild birds and very contagious among both domestic (POULTRY) and wild birds. It does not usually infect humans, but some cases have been reported.	H5N1 Avian Influenza Virus,H5N1 Bird Flu Virus,H5N1 Virus,H5N1 subtype,H5N1 Viruses,H5N1 subtypes,Virus, H5N1
D055664	Electrochemical Techniques	The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.	Electrochemical Technics,Electrochemical Technic,Electrochemical Technique,Technic, Electrochemical,Technics, Electrochemical,Technique, Electrochemical,Techniques, Electrochemical
D058525	Quartz Crystal Microbalance Techniques	The use of a quartz crystal microbalance for measuring weights and forces in the micro- to nanogram range. It is used to study the chemical and mechanical properties of thin layers, such as polymer coatings and lipid membranes; and interactions between molecues.	Electrochemical Quartz Crystal Impedance Analysis,Quartz Crystal Microbalance,Quartz Crystal Microbalance with Dissipation Monitoring,Microbalance, Quartz Crystal,Microbalances, Quartz Crystal,Quartz Crystal Microbalances
D020136	Hydrogel, Polyethylene Glycol Dimethacrylate	A network of cross-linked hydrophilic macromolecules used in biomedical applications fabricated by photopolymerization of polyethylene glycol dimethacrylate. Its general formulae is C3H5C(O)(OCH2CH2)nOC(O)C3H5 where n denotes a number of average polyglycol (OCH2CH2) repeats.	PEG-DMA Hydrogel,PEGDMA Hydrogel,Polyethylene Glycol Dimethacrylate Hydrogel,Hydrogel, PEG-DMA,Hydrogel, PEGDMA,PEG DMA Hydrogel,PEG-DMA Hydrogels,PEGDMA Hydrogels