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To verify the influence of the protein binding status of steroids adjacent to adrenal cells on steroidogenesis, the effect of transcortin, a specific binding protein of cortisol or corticosterone, on adrenocorticotropin (ACTH)-stimulated corticosterone production in monolayer cultured rat adrenal cells was studied. The transcortin in concentration of 5 x 10(-7) M was loaded with 0, 2.5, 5 and 10 pg/ml ACTH-(1-24), and the cells were incubated for 2 and 4 hours. Since molar concentrations of corticosterone produced in the medium were below the transcortin concentration at all levels of stimulation, protein-unbound corticosterone in the medium may have been largely reduced by the addition of transcortin. However, the total corticosterone production was not influenced by the transcortin added to the medium. It was speculated that protein-unbound steroid within the concentration range modulated by transcortin in the area surrounding the adrenal cells may not affect adrenal steroidogenesis.
K Ogawa, and
N Matsui
October 1973,
FEBS letters,
K Ogawa, and
N Matsui
April 1990,
Endocrinology,
K Ogawa, and
N Matsui
December 1983,
Endocrinology,
K Ogawa, and
N Matsui
April 1999,
The Journal of pharmacy and pharmacology,
K Ogawa, and
N Matsui
September 1970,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
K Ogawa, and
N Matsui
July 1978,
Biochimica et biophysica acta,
K Ogawa, and
N Matsui
January 1968,
Annales d'endocrinologie,
K Ogawa, and
N Matsui
September 1987,
Endocrinology,
K Ogawa, and
N Matsui
December 1990,
Hypertension (Dallas, Tex. : 1979),
K Ogawa, and
N Matsui
September 1984,
Life sciences,
