A quantitative method for maceration of hydra tissue. 1973

Charles N David
Max-Planck-Institut fÜr Virusforschung, 7400, TÜbingen, Germany.

A method is described for the maceration (dissociation) of hydra tissue into single cells. The cells have characteristic morphology such that all basic types - epithelial, gland, mucous, interstitial, nematoblast, and nerve - can be distinguished. Criteria are given for identifying each cell type by phase contrast microscopy. It is shown that maceration quantitatively recovers cells from hydra tissue.

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