Biomaterial Database

Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection with Staphylococcus aureus in a Model Air Pouch. 2017

Hellen Braga Martins, and Nathan das Neves Selis, and Clarissa Leal Silva E Souza, and Flávia S Nascimento, and Suzi Pacheco de Carvalho, and Lorena D'Oliveira Gusmão, and Jannine Dos Santos Nascimento, and Anne Karoline Pereira Brito, and Samira Itana de Souza, and Marcio Vasconcelos de Oliveira, and Jorge Timenetsky, and Regiane Yatsuda, and Ana Paula T Uetanabaro, and Lucas M Marques

State University of Santa Cruz (UESC), Campus Soane Nazaré de Andrade, Ilhéus, BA, Brazil.

This study proposes to implement an alternative and effective strategy for local treatment of disease provoked by S. aureus. For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal's blood, and cytokines IL-1β, IL-6, and TNF-α were titrated using ELISA in the air pouch lavage. Moreover, TNF-α, IL-1β, and IL-6 gene expression was analyzed through an RT-qPCR array, and S. aureus was quantified using qPCR. Our results, p < 0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF-α levels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, and S. aureus. Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.