Biomaterial Database

A modulating role of prostaglandins in catecholamine release by perfused dog adrenal glands. 1988

S Yamada, and K Morita, and T Dohi, and A Tsujimoto

Department of Pharmacology, Hiroshima University School of Dentistry, Japan.

PGE2, PGE1, PGF2 alpha and PGD2 dose dependently potentiated the ACh-evoked catecholamine (CA) release from perfused dog adrenal glands. These prostaglandins by themselves slightly stimulated CA release. PGE2 potentiated the CA release induced by stimulation of nicotinic or muscarinic cholinergic receptors and also by stimulation with excess K+ or caffeine. However, PGE2 did not affect the release induced by caffeine in the absence of extracellular Ca2+. ACh stimulated the 45Ca efflux from adrenals prelabelled with 45Ca. PGE2 enhanced the ACh-stimulated 45Ca efflux. The CA release in response to ACh or nicotine was slightly but significantly reduced by pretreatment of the adrenals with indomethacin. SC19220, a specific prostaglandin antagonist significantly reduced the ACh-evoked CA release and blocked the enhancing action of PGE2 on the stimulation-evoked CA release. The results showed that prostaglandins stimulated the CA release evoked in perfused dog adrenals and that the effect on the Ca2+ flux across the plasma membrane could be involved in the prostaglandin mechanism of action. The possibility is suggested that prostaglandins formed endogenously in response to physiological stimulation of chromaffin cells could play a facilitating, modulatory role in CA release.

UI	MeSH Term	Description	Entries
D007213	Indomethacin	A non-steroidal anti-inflammatory agent (NSAID) that inhibits CYCLOOXYGENASE, which is necessary for the formation of PROSTAGLANDINS and other AUTACOIDS. It also inhibits the motility of POLYMORPHONUCLEAR LEUKOCYTES.	Amuno,Indocid,Indocin,Indomet 140,Indometacin,Indomethacin Hydrochloride,Metindol,Osmosin
D008297	Male		Males
D010477	Perfusion	Treatment process involving the injection of fluid into an organ or tissue.	Perfusions
D011453	Prostaglandins	A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.	Prostaglandin,Prostanoid,Prostanoids
D011458	Prostaglandins E	(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.	PGE
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002132	Calcium Radioisotopes	Unstable isotopes of calcium that decay or disintegrate emitting radiation. Ca atoms with atomic weights 39, 41, 45, 47, 49, and 50 are radioactive calcium isotopes.	Radioisotopes, Calcium
D002395	Catecholamines	A general class of ortho-dihydroxyphenylalkylamines derived from TYROSINE.	Catecholamine,Sympathin,Sympathins
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D005260	Female		Females