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The association of topoisomerase II enzymes with papovavirus-chromatin has been described recently. We used cells infected with simian virus 40 (SV40) to investigate the in vivo effect of the topoisomerase II inhibitors nalidixic acid and novobiocin on the viral chromatin template. Blocking of topoisomerases inhibits DNA and RNA synthesis. The block with the inhibitors resulted in a conversion of a 95- to 75-svedberg chromatin to a 180- to 150-svedberg component accompanied with a variation in the topological linking number of the DNA. The condensed chromatin fractions (180-150 S) from inhibited cells resemble encapsidation intermediates and their DNA has a linking number as the DNA extracted from purified virions. Moreover, the virion DNA has a higher superhelical density compared to intracellular chromatin isolated from untreated cells and argues for a supercoiling activity in the cell.
W Waldeck, and
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F Rösl
August 1974,
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W Waldeck, and
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F Rösl
August 1984,
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W Waldeck, and
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F Rösl
December 1986,
Molecular and cellular biology,
W Waldeck, and
H Zentgraf, and
F Rösl
January 1987,
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W Waldeck, and
H Zentgraf, and
F Rösl
August 1981,
The Journal of biological chemistry,
W Waldeck, and
H Zentgraf, and
F Rösl
July 1980,
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W Waldeck, and
H Zentgraf, and
F Rösl
July 1973,
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W Waldeck, and
H Zentgraf, and
F Rösl
August 1985,
Molecular and cellular biology,
W Waldeck, and
H Zentgraf, and
F Rösl
March 1999,
Molecular and cellular biology,
W Waldeck, and
H Zentgraf, and
F Rösl
July 1983,
Virology,
