Biomaterial Database

Evidence that phosphoinositides mediate motility in cochlear outer hair cells. 1987

J Schacht, and H P Zenner

Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109.

Cochlear outer hair cells are postulated to modulate auditory transduction by their mechanical properties which presumably are controlled by efferent neurons and regulated by the levels of intracellular calcium. In a number of biological systems these calcium levels are controlled by inositol trisphosphate (InsP3), the second messenger of the phosphoinositide cascade. We have investigated whether the phosphoinositides function in the signal transfer in mammalian auditory sensory cells. Live isolated outer hair cells synthesize the lipids of the phosphoinositide cycle, phosphatidic acid (Ptd), phosphatidylinositol (PtdIns), phosphatidylinositol 4-phosphate (PtdInsP) and phosphatidylinositol 4,5-bisphosphate (PtdInsP2). After one hour of incubation with [32P]orthophosphate, Ptd constituted 21 +/- 7% of labeled lipids, PtdIns 19 +/- 5%, PtdInsP 28 +/- 7% and PtdInsP2 33 +/- 7%. Contractions were elicited in isolated cells after their membrane was permeabilized with Triton X-100. Addition of calcium and ATP resulted in contractions (average, 7.2% of initial cell length) in 81% of the cells studied; the omission of ATP or calcium (i.e. the presence of the calcium-chelator EGTA) reduced the incidence of contractions to 0% and 17%, respectively. In the absence of calcium, the putative second messenger, InsP3, caused contractions in 63% of the observed cells while the physiologically inactive agents inositol and inositol bisphosphate failed to elicit responses. The results are compatible with the suggestion that InsP3 mediates contractility in outer hair cells.

UI	MeSH Term	Description	Entries
D010716	Phosphatidylinositols	Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.	Inositide Phospholipid,Inositol Phosphoglyceride,Inositol Phosphoglycerides,Inositol Phospholipid,Phosphoinositide,Phosphoinositides,PtdIns,Inositide Phospholipids,Inositol Phospholipids,Phosphatidyl Inositol,Phosphatidylinositol,Inositol, Phosphatidyl,Phosphoglyceride, Inositol,Phosphoglycerides, Inositol,Phospholipid, Inositide,Phospholipid, Inositol,Phospholipids, Inositide,Phospholipids, Inositol
D002465	Cell Movement	The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.	Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006198	Hair Cells, Auditory	Sensory cells in the organ of Corti, characterized by their apical stereocilia (hair-like projections). The inner and outer hair cells, as defined by their proximity to the core of spongy bone (the modiolus), change morphologically along the COCHLEA. Towards the cochlear apex, the length of hair cell bodies and their apical STEREOCILIA increase, allowing differential responses to various frequencies of sound.	Auditory Hair Cells,Cochlear Hair Cells,Auditory Hair Cell,Cell, Cochlear Hair,Cells, Cochlear Hair,Cochlear Hair Cell,Hair Cell, Auditory,Hair Cell, Cochlear,Hair Cells, Cochlear
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In