BIOMAT Database Logo BIOMAT Database Logo

Non-radioactive in situ hybridization for the detection of cytomegalovirus infections. 1988

A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
Department of Cytochemistry and Cytometry, University of Leiden, The Netherlands.

Acetylaminofluorene (AAF) modified cytomegalovirus (CMV) DNA probes have been applied for the rapid detection of CMV genomes by non-radioactive in situ hybridization in routinely obtained pathological material. To establish proper protocols, AAF modified mouse satellite DNA and mouse liver were used to investigate the procedural variables. Among these were type and time of fixation, glass slide coating for improved tissue adherence, protease permeabilization of sections, type and time of denaturation and hybridization, probe concentration, post-hybridization washing conditions and immunocytochemical detection. This research has led to a user-friendly procedure which, in addition to cells displaying a cytopathological effect typical for CMV infection, detects with high sensitivity CMV carrying cells that show no histo-pathological alterations. It can be readily applied in routine clinical-diagnostic laboratories.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D003586 Cytomegalovirus Infections Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults. CMV Inclusion,CMV Inclusions,Congenital CMV Infection,Congenital Cytomegalovirus Infection,Cytomegalic Inclusion Disease,Cytomegalovirus Colitis,Cytomegalovirus Inclusion,Cytomegalovirus Inclusion Disease,Cytomegalovirus Inclusions,Inclusion Disease,Perinatal CMV Infection,Perinatal Cytomegalovirus Infection,Renal Tubular Cytomegalovirus Inclusion,Renal Tubular Cytomegalovirus Inclusions,Salivary Gland Virus Disease,Severe Cytomegalovirus Infection,Severe Cytomegalovirus Infections,Infections, Cytomegalovirus,CMV Infection, Congenital,CMV Infection, Perinatal,Colitis, Cytomegalovirus,Congenital CMV Infections,Congenital Cytomegalovirus Infections,Cytomegalic Inclusion Diseases,Cytomegalovirus Colitides,Cytomegalovirus Inclusion Diseases,Cytomegalovirus Infection,Cytomegalovirus Infection, Congenital,Cytomegalovirus Infection, Perinatal,Cytomegalovirus Infection, Severe,Cytomegalovirus Infections, Severe,Disease, Cytomegalic Inclusion,Disease, Cytomegalovirus Inclusion,Diseases, Cytomegalovirus Inclusion,Inclusion Disease, Cytomegalic,Inclusion Disease, Cytomegalovirus,Inclusion Diseases,Inclusion Diseases, Cytomegalovirus,Inclusion, CMV,Inclusion, Cytomegalovirus,Infection, Congenital CMV,Infection, Congenital Cytomegalovirus,Infection, Cytomegalovirus,Infection, Perinatal CMV,Infection, Perinatal Cytomegalovirus,Infection, Severe Cytomegalovirus,Perinatal CMV Infections,Perinatal Cytomegalovirus Infections
D003587 Cytomegalovirus A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS. Herpesvirus 5, Human,Human Herpesvirus 5,Salivary Gland Viruses,HHV 5,Herpesvirus 5 (beta), Human,Cytomegaloviruses,Salivary Gland Virus,Virus, Salivary Gland,Viruses, Salivary Gland
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015073 2-Acetylaminofluorene A hepatic carcinogen whose mechanism of activation involves N-hydroxylation to the aryl hydroxamic acid followed by enzymatic sulfonation to sulfoxyfluorenylacetamide. It is used to study the carcinogenicity and mutagenicity of aromatic amines. 2-Acetamidofluorene,Fluoren-2-ylacetamide,2-AAF,2-Fluorenylacetamide,AAF, Aminofluorene,Acetylaminofluorene,N-2-Fluorenylacetamide,N-Acetyl-2-Aminofluorene,2 Acetamidofluorene,2 Acetylaminofluorene,2 Fluorenylacetamide,Aminofluorene AAF,Fluoren 2 ylacetamide,N 2 Fluorenylacetamide,N Acetyl 2 Aminofluorene
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
RNA detection using non-radioactive in situ hybridization.
February 1995, Current opinion in biotechnology,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
Double in situ hybridization for detection of Herpes simplex virus and cytomegalovirus DNA using non-radioactive probes.
March 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
In situ hybridization for detection of cytomegalovirus.
July 1990, Archives of pathology & laboratory medicine,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
Chemiluminescent in situ hybridization for the detection of cytomegalovirus DNA.
April 1996, The American journal of pathology,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
An improved, sensitive, non-radioactive in situ hybridization method for the detection of cytokine mRNAs.
May 1995, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry.
August 1992, Histochemistry,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
[Selective detection of cytomegalovirus by in situ hybridization].
January 1988, Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
In situ hybridization and immunohistochemistry for the detection of porcine cytomegalovirus.
January 2012, Journal of virological methods,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
Detection of Leishmania parasites by DNA in situ hybridization with non-radioactive probes.
January 1987, Parasitology research,
A K Raap, and J L Geelen, and J W van der Meer, and F M van de Rijke, and P van den Boogaart, and M van der Ploeg
Bi-color detection of two target DNAs by non-radioactive in situ hybridization.
January 1986, Histochemistry,
Copied contents to your clipboard!