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The gene for human interleukin-1 beta was cloned from SK-hep-1 hepatoma cellular RNA and expressed at high levels in Escherichia coli both as the naturally processed form (rIL-1 beta) and as a variant with an additional sequence of three amino acids on the N-terminus (rIL-1 beta +). Expressed protein was purified to homogeneity by a sequence of steps, which included low pH incubation, adsorption and desorption from Procion Red Sepharose, sizing on a Superose 12 fast-performance liquid chromatography (FPLC) column, and anion exchange chromatography on QAE Sepharose. The final step provided a biologically active protein that migrates on two-dimensional (2-D) gels as a single spot with a pI of 6.7 +/- 0.2 and a molecular mass of 17,500 daltons. Concentrated solutions of rIL-1 beta have produced crystals by ammonium sulfate precipitation. The crystals are tetragonal, show the symmetry of space group P4(1) or its enantiomer, have lattice constants of a = 58.46 (1) and c = 77.02 (3) A, and scatter to at least 2 A resolution. A structure determination based on these crystals is under way.
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
October 1987,
The Journal of biological chemistry,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
May 1989,
Journal of leukocyte biology,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
November 1989,
FEBS letters,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
August 1987,
The Journal of biological chemistry,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
September 1986,
Immunobiology,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
February 1986,
Methods and findings in experimental and clinical pharmacology,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
February 1990,
Scandinavian journal of immunology,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
May 1992,
Biological chemistry Hoppe-Seyler,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
August 1989,
Inflammation,
D B Carter, and
K A Curry, and
C S Tomich, and
A W Yem, and
M R Deibel, and
D E Tracey, and
J W Paslay, and
J B Carter, and
N Y Theriault, and
P K Harris
January 1989,
The Journal of biological chemistry,
