Biomaterial Database

A comparison of infectious bronchitis virus hemagglutination-inhibition test procedures. 1988

D J King

U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.

Replicate hemagglutination-inhibition (HI) tests of selected convalescent and hyperimmune infectious bronchitis virus (IBV) antisera were run against five IBV antigens: Massachusetts 41, Connecticut 46, H52, Arkansas 99, and JMK. Six different HI test procedures were included in this comparison to evaluate the effect of procedural differences on HI titers. The results were similar among procedures to the extent that positive and negative reactions were differentiated by each procedure with each antigen. This indicated that specificity was not affected by the procedure. However, the mean titers did differ among procedures in tests of the same serum-antigen combination, and the range of the titer differences was frequently fourfold or greater. Generally, high or low titers were associated with specific procedures, regardless of which test antigen was used.

UI	MeSH Term	Description	Entries
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003332	Coronaviridae	Spherical RNA viruses, in the order NIDOVIRALES, infecting a wide range of animals including humans. Transmission is by fecal-oral and respiratory routes. Mechanical transmission is also common. There are two genera: CORONAVIRUS and TOROVIRUS.	Bafinivirus,Coronavirinae,Torovirinae,White bream virus,Bafiniviruses,White bream viruses
D006385	Hemagglutination Inhibition Tests	Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.	Hemagglutination Inhibition Test,Inhibition Test, Hemagglutination,Inhibition Tests, Hemagglutination,Test, Hemagglutination Inhibition,Tests, Hemagglutination Inhibition
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D001351	Infectious bronchitis virus	A species of CORONAVIRUS causing infections in chickens and possibly pheasants. Chicks up to four weeks old are the most severely affected.	Avian Infectious Bronchitis Virus,Infectious Bronchitis Virus, Avian,Infectious bronchitis viruses,bronchitis viruses, Infectious