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Using liver from nonadrenalectomized adult male rats, binding sites for [3H]dexamethasone in particulate fractions are demonstrated. The binding is thermolabile, saturable, and specific for glucocorticoid. The apparent dissociation constant (Kdapp) for [3H]dexamethasone (0.48 +/- 0.084 microM) is 60-fold greater than that for cytosolic receptor (7.9 +/- 1.5 nM). The Kdapp for [3H]cortisol in particulate fractions is 2.5-fold lower than for [3H]dexamethasone (Kdapp = 0.18 microM). The binding capacities for particulate and cytosolic glucocorticoid-binding sites also differ significantly, with particulate sites at least 9.1-fold more concentrated than cytosolic sites in liver tissue. Particulate sites are determined in Percoll density gradients to have a density of 1.039 g/cc. Saturable [3H]dexamethasone radioactivity coelutes from these gradients with the plasma membrane marker enzyme 5'-nucleotidase. Adrenalectomy causes the complete loss of particulate binding sites by 6 days postadrenalectomy; however, these sites can be regenerated to two thirds of the nonadrenalectomy level by 20-30 days postadrenalectomy.
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
January 1976,
Recent advances in studies on cardiac structure and metabolism,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
June 1975,
Canadian journal of physiology and pharmacology,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
October 1994,
Sheng li xue bao : [Acta physiologica Sinica],
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
November 1981,
Plant physiology,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
September 1987,
Japanese journal of pharmacology,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
May 1985,
The Journal of biological chemistry,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
September 1980,
Journal of molecular and cellular cardiology,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
October 1977,
Photochemistry and photobiology,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
December 1980,
Plant physiology,
F W Quelle, and
R V Smith, and
C A Hrycyna, and
T D Kaliban, and
J A Crooks, and
J M O'Brien
January 1993,
Sbornik lekarsky,
