-transparent.png){kind=logo}
A method of fractionating proteins by high-performance liquid partition chromatography has been developed and used for isolation and purification to homogeneity of one of the species of human leukocyte interferon. The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Extraction of the gel gave a single sharp peak of antiviral activity coinciding with the protein band. The specific activity of pure interferon was found to be 2--4 X 10(8) units/mg, based on amino acid analysis. The molecular weight is 17,500--18,000.
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
January 1974,
Japanese journal of microbiology,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
November 1984,
Biochemistry,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
August 1976,
The Journal of biological chemistry,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
January 1974,
In vitro. Monograph,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
February 1976,
Proceedings of the National Academy of Sciences of the United States of America,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
August 1987,
The Journal of biological chemistry,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
June 1992,
Journal of chromatography,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
September 1981,
The Journal of biological chemistry,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
April 1988,
Journal of interferon research,
M Rubinstein, and
S Rubinstein, and
P C Familletti, and
R S Miller, and
A A Waldman, and
S Pestka
January 1980,
The Journal of general virology,
