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In experiments on human and rat platelets the changes in cytoplasmic pH (pHi) and Ca2+ concentration (Ca2+) have been studied by the use of fluorescent probes BCECF and quin-2, respectively. Inhibition of Na+/H+ exchange resulted in removal of external Na+ (equimolar substitution by cholin) induced a considerable reduction of Ca2+-signal caused by 10 mMPAF, and a slight decrease in Ca2+-signal elicited by 0.1 mu/ml thrombin. In the control Na+ and Ca2+ containing medium both PAF and thrombin induced first a decrease then an increase of pHi above its original level. The latter phase being much more pronounced in the case of thrombin action. Removal of Ca2+ from the external solution suppressed pHi increase and correspondingly it enhanced initial decrease. Addition of Ni2+ also suppressed stimulus-induced pHi increase. A treatment of platelets by Ca-ionophore A23187 caused a rise of pHi without its initial decrease; in medium without Ca2+ the changes of pHi were inhibited. The results obtained suggest that in platelets there exist a mutual interdependence between Ca2+ influx and change in pHi: Ca2+ influx enhanced the activation of Na+/H+ exchange by agonist; in turn Na+/H+ exchange activation enhances the stimulus-induced Ca2+ influx.
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
January 1988,
Progress in clinical and biological research,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
April 1988,
Trends in biochemical sciences,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
November 1983,
The Journal of general physiology,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
October 1983,
Proceedings of the National Academy of Sciences of the United States of America,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
December 1991,
Journal of hypertension. Supplement : official journal of the International Society of Hypertension,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
January 1991,
Annals of the New York Academy of Sciences,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
February 1987,
Circulation research,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
December 1990,
The American journal of physiology,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
April 1994,
Pulmonary pharmacology,
M L Borin, and
V G Pinelis, and
S S Bykov, and
Iu V Kudinov, and
O A Azizova
January 1996,
Bulletin et memoires de l'Academie royale de medecine de Belgique,
