BIOMAT Database Logo BIOMAT Database Logo

Amino acid substitutions and alteration in cation specificity in the melibiose carrier of Escherichia coli. 1988

T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.

We isolated mutants of Escherichia coli which showed Li+-resistant growth on melibiose. The melibiose carrier of the mutants lost the ability to couple to H+, whereas it retained the ability to couple to Na+. The mutated gene, melB, of the mutants was cloned, and the nucleotide sequence was determined. The nucleotide replacements caused the following substitutions of amino acid residues in the melibiose carrier: Pro-142 with Ser, Leu-232 with Phe, or Ala-236 with Thr or Val. These amino acid residues are located in slightly hydrophobic regions of the melibiose carrier. The results provide strong support for the idea that such regions or their vicinities which contain those amino acid residues play an important role in H+ (or Li+) recognition or H+ (or Li+) transport by the melibiose carrier.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008094 Lithium An element in the alkali metals family. It has the atomic symbol Li, atomic number 3, and atomic weight [6.938; 6.997]. Salts of lithium are used in treating BIPOLAR DISORDER. Lithium-7,Lithium 7
D008553 Melibiose A disaccharide consisting of one galactose and one glucose moiety in an alpha (1-6) glycosidic linkage.
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D002712 Chlorides Inorganic compounds derived from hydrochloric acid that contain the Cl- ion. Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial

Related Publications

T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Cation specificity for sugar substrates of the melibiose carrier in Escherichia coli.
November 1987, Biochimica et biophysica acta,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Alteration in cation specificity of the melibiose transport carrier of Escherichia coli due to replacement of proline 122 with serine.
June 1985, Journal of bacteriology,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Mutations that simultaneously alter both sugar and cation specificity in the melibiose carrier of Escherichia coli.
September 1988, The Journal of biological chemistry,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Escherichia coli mutants with altered cation recognition by the melibiose carrier.
October 1984, Journal of bacteriology,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
The melibiose carrier of Escherichia coli.
January 1990, Research in microbiology,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
The melibiose carrier of Escherichia coli: cysteine substitutions for individual residues in helix XI.
March 2000, The Journal of membrane biology,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Cysteine substitutions for individual residues in helix VI of the melibiose carrier of Escherichia coli.
September 2001, The Journal of membrane biology,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Melibiose-cation cotransport system of Escherichia coli.
January 1985, Annals of the New York Academy of Sciences,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Reconstitution of the melibiose carrier of Escherichia coli.
January 1985, Membrane biochemistry,
T Kawakami, and Y Akizawa, and T Ishikawa, and T Shimamoto, and M Tsuda, and T Tsuchiya
Cysteine mutagenesis of the amino acid residues of transmembrane helix I in the melibiose carrier of Escherichia coli.
May 2001, Biochemistry,
Copied contents to your clipboard!