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Construction of a map-based reference genome sequence for barley, Hordeum vulgare L. 2017
Sebastian Beier, and
Axel Himmelbach, and
Christian Colmsee, and
Xiao-Qi Zhang, and
Roberto A Barrero, and
Qisen Zhang, and
Lin Li, and
Micha Bayer, and
Daniel Bolser, and
Stefan Taudien, and
Marco Groth, and
Marius Felder, and
Alex Hastie, and
Hana Šimková, and
Helena Staňková, and
Jan Vrána, and
Saki Chan, and
María Muñoz-Amatriaín, and
Rachid Ounit, and
Steve Wanamaker, and
Thomas Schmutzer, and
Lala Aliyeva-Schnorr, and
Stefano Grasso, and
Jaakko Tanskanen, and
Dharanya Sampath, and
Darren Heavens, and
Sujie Cao, and
Brett Chapman, and
Fei Dai, and
Yong Han, and
Hua Li, and
Xuan Li, and
Chongyun Lin, and
John K McCooke, and
Cong Tan, and
Songbo Wang, and
Shuya Yin, and
Gaofeng Zhou, and
Jesse A Poland, and
Matthew I Bellgard, and
Andreas Houben, and
Jaroslav Doležel, and
Sarah Ayling, and
Stefano Lonardi, and
Peter Langridge, and
Gary J Muehlbauer, and
Paul Kersey, and
Matthew D Clark, and
Mario Caccamo, and
Alan H Schulman, and
Matthias Platzer, and
Timothy J Close, and
Mats Hansson, and
Guoping Zhang, and
Ilka Braumann, and
Chengdao Li, and
Robbie Waugh, and
Uwe Scholz, and
Nils Stein, and
Martin Mascher
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 06466 Seeland, Germany.
Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. 'Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).
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Axel Himmelbach, and
Christian Colmsee, and
Xiao-Qi Zhang, and
Roberto A Barrero, and
Qisen Zhang, and
Lin Li, and
Micha Bayer, and
Daniel Bolser, and
Stefan Taudien, and
Marco Groth, and
Marius Felder, and
Alex Hastie, and
Hana Šimková, and
Helena Staňková, and
Jan Vrána, and
Saki Chan, and
María Muñoz-Amatriaín, and
Rachid Ounit, and
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Sujie Cao, and
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Axel Himmelbach, and
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Xiao-Qi Zhang, and
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Qisen Zhang, and
Lin Li, and
Micha Bayer, and
Daniel Bolser, and
Stefan Taudien, and
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Hua Li, and
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Uwe Scholz, and
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Qisen Zhang, and
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Hua Li, and
Xuan Li, and
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Songbo Wang, and
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Gaofeng Zhou, and
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Sebastian Beier, and
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Daniel Bolser, and
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Jan Vrána, and
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María Muñoz-Amatriaín, and
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Plant physiology,
Sebastian Beier, and
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Mario Caccamo, and
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Martin Mascher
July 2015,
Genetika,
