Biomaterial Database

Remarkably homogeneous population of adenovirus type 3 and 7 genome types. 1988

M Arens, and V Dilworth

Department of Pediatrics/Adolescent Medicine, St. Louis University Medical School, Missouri 63104.

A total of 270 frozen adenovirus isolates, obtained from January 1981 to December 1986, were grown in KB cells. DNA was successfully prepared from 248 of these isolates, and the prepared DNA was digested initially with SmaI restriction endonuclease and then analyzed by agarose gel electrophoresis. Restriction fragment patterns showed that 68 of these DNAs were either adenovirus type 3 (Ad3) or Ad7. Further analysis by digestion with BamHI showed that 24 isolates were Ad3a and 44 isolates were Ad7b. When Ad3 isolates were digested with SmaI or PstI, the restriction fragment patterns of the 24 isolates were identical. Of these 24 isolates, 20 (83%) also gave identical patterns when digested with HindIII, and the patterns of the remaining 4 isolates were different from that of the 20 but identical to each other. All Ad3 isolates were obtained in 1982, 1983, and 1986. All the 44 Ad7b isolates gave identical fragment patterns when digested with BglI or PvuII, and 43 of 44 (98%) isolates gave identical patterns when digested with SstII. We obtained at least two Ad7 isolates during each of the 6 years studied. Although considerable genome heterogeneity within adenovirus serotypes and subtypes has been reported, our population of isolates is remarkably homogeneous. This homogeneous set of genome types was obtained from diverse anatomical sites and was associated with a broad spectrum of clinical syndromes.

UI	MeSH Term	Description	Entries
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D004262	DNA Restriction Enzymes	Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.	Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D004587	Electrophoresis, Agar Gel	Electrophoresis in which agar or agarose gel is used as the diffusion medium.	Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D005838	Genotype	The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.	Genogroup,Genogroups,Genotypes
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000260	Adenoviruses, Human	Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G.	APC Viruses,APC Virus,Adenovirus, Human,Human Adenovirus,Human Adenoviruses