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We investigated the effect of retinoic acid (RA) and herbimycin A (herb-A) on cell growth, cell differentiation, and colony formation of human neuroblastoma cell lines. The neuroblastoma line SK-N-SH expressed both neuroblast and nonneuronal phenotypes, whereas its subclone SH-SY5Y and the Kelly cell line were predominantly neuroblastic. Both herb-A and RA, given alone, moderately reduced cell growth and colony formation of the neuroblastic cell lines. Growth curve analyses with SK-N-SH suggested that herb-A greatly reduced the number of initially growing cells, whereas RA slightly enhanced initial cell growth. Morphological changes were determined with the use of rhodaminephalloidin staining of actin. Retinoic acid caused an increase in the fraction of neuroblast cell in SK-N-SH, and conversely of nonneuronal cells in SH-SY5Y and Kelly cell lines. Both drugs also caused partial differentiation towards a neuronal phenotype, and herb-A induced selective lysis of nonneuronal cells of SK-N-SH. Because of their discrepant effects, RA (10 microM) and herb-A (236 nM) were tested in combination at a concentration that had only moderate effects when given alone. The combination further reduced cell growth and colony formation and dramatically enhanced differentiation towards a neuronal morphology. The Kelly cell line with amplified N-myc genome, which correlates with clinical progression of neuroblastoma, was also sensitive to RA plus herb-A. These results recommend the combination of RA and herb-A for differentiation therapy of neuroblastoma.
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
July 1997,
European journal of cell biology,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
January 1988,
Progress in clinical and biological research,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
February 1998,
Biochemical and biophysical research communications,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
July 2023,
Journal of proteome research,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
August 2015,
Molecular cancer,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
January 1992,
Journal of nutritional science and vitaminology,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
April 2016,
Neurochemical research,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
June 1984,
Cell differentiation,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
January 1991,
Anticancer research,
P N Preis, and
H Saya, and
L Nádasdi, and
G Hochhaus, and
V Levin, and
W Sadée
September 1992,
Experimental cell research,
