Physiological functions of pyruvate:NADP+ oxidoreductase and 2-oxoglutarate decarboxylase in Euglena gracilis under aerobic and anaerobic conditions. 2017

Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
a Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.

In Euglena gracilis, pyruvate:NADP+ oxidoreductase, in addition to the pyruvate dehydrogenase complex, functions for the oxidative decarboxylation of pyruvate in the mitochondria. Furthermore, the 2-oxoglutarate dehydrogenase complex is absent, and instead 2-oxoglutarate decarboxylase is found in the mitochondria. To elucidate the central carbon and energy metabolisms in Euglena under aerobic and anaerobic conditions, physiological significances of these enzymes involved in 2-oxoacid metabolism were examined by gene silencing experiments. The pyruvate dehydrogenase complex was indispensable for aerobic cell growth in a glucose medium, although its activity was less than 1% of that of pyruvate:NADP+ oxidoreductase. In contrast, pyruvate:NADP+ oxidoreductase was only involved in the anaerobic energy metabolism (wax ester fermentation). Aerobic cell growth was almost completely suppressed when the 2-oxoglutarate decarboxylase gene was silenced, suggesting that the tricarboxylic acid cycle is modified in Euglena and 2-oxoglutarate decarboxylase takes the place of the 2-oxoglutarate dehydrogenase complex in the aerobic respiratory metabolism.

UI MeSH Term Description Entries
D007658 Ketone Oxidoreductases Oxidoreductases that are specific for KETONES. Oxidoreductases, Ketone
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008928 Mitochondria Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed) Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D010084 Oxidation-Reduction A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471). Redox,Oxidation Reduction
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002262 Carboxy-Lyases Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1. Carboxy-Lyase,Decarboxylase,Decarboxylases,Carboxy Lyase,Carboxy Lyases
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D003653 Decarboxylation The removal of a carboxyl group, usually in the form of carbon dioxide, from a chemical compound. Decarboxylations
D004734 Energy Metabolism The chemical reactions involved in the production and utilization of various forms of energy in cells. Bioenergetics,Energy Expenditure,Bioenergetic,Energy Expenditures,Energy Metabolisms,Expenditure, Energy,Expenditures, Energy,Metabolism, Energy,Metabolisms, Energy

Related Publications

Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
July 1987, The Journal of biological chemistry,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
November 1989, Archives of biochemistry and biophysics,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
July 1991, Archives of biochemistry and biophysics,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
March 1992, The Biochemical journal,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
May 2004, The Journal of biological chemistry,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
April 1991, Archives of biochemistry and biophysics,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
March 2003, Archives of biochemistry and biophysics,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
February 1974, Plant physiology,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
August 1990, Archives of biochemistry and biophysics,
Masami Nakazawa, and Ryuta Hayashi, and Shigeo Takenaka, and Hiroshi Inui, and Takahiro Ishikawa, and Mitsuhiro Ueda, and Tatsuji Sakamoto, and Yoshihisa Nakano, and Kazutaka Miyatake
May 2015, Journal of hazardous materials,
Copied contents to your clipboard!