-transparent.png){kind=logo}
We have demonstrated that precise excision of bacterial transposon Tn5 can occur in the yeast, Saccharomyces cerevisiae. Tn5 insertions in the yeast gene LYS2 were generated by transposon mutagenesis made in Escherichia coli by means of a lambda::Tn5 vector. Nine insertions of Tn5 into the structural part of the yeast LYS2 gene situated in a shuttle episomal plasmid were selected. All the plasmids with a Tn5 insertion were used to transform yeast strains carrying a deletion of the entire LYS2 gene or a deletion of the part of LYS2 overlapping the point of insertion. All insertions inactivated the LYS2 gene and were able to revert with low (about 10(-8) frequencies to lysine prototrophy. Restriction analysis of revertant plasmids revealed them to be indistinguishable from the original plasmid without Tn5 insertion. DNA sequencing of the regions containing the points of insertions, made for two revertants, proved that Tn5 excision was completely precise.
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
May 1990,
Journal of general microbiology,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
September 1984,
Molecular biology and evolution,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
December 1983,
Proceedings of the National Academy of Sciences of the United States of America,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
April 1983,
Gene,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
May 1992,
Proceedings of the National Academy of Sciences of the United States of America,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
January 2008,
Annual review of genetics,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
January 1993,
Annual review of microbiology,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
April 1989,
Mutation research,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
January 1981,
Proceedings of the National Academy of Sciences of the United States of America,
D A Gordenin, and
M V Trofimova, and
O N Shaburova, and
Y I Pavlov, and
Y O Chernoff, and
Y V Chekuolene, and
Y Y Proscyavichus, and
K V Sasnauskas, and
A A Janulaitis
December 1983,
Genetics,
