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Detection of Cholera Toxin by an Immunochromatographic Test Strip. 2017
Eiki Yamasaki, and
Ryuta Sakamoto, and
Takashi Matsumoto, and
Biswajit Maiti, and
Kayo Okumura, and
Fumiki Morimatsu, and
G Balakrish Nair, and
Hisao Kurazono
Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan. yamasakie@obihiro.ac.jp.
As cholera toxin (CT) is responsible for most of the symptoms induced by Vibrio cholerae O1 or O139 infection, detection of CT is an important biomarker for diagnosis of the disease. The procedure for pathogenicity analysis of V. cholerae isolates must be carefully developed for the reason that the amount of CT produced by V. cholerae varies according to the medium used and culture conditions (i.e. temperature and aeration status) applied. Here we describe a reproducible rapid method for analysis of CT production by toxigenic V. cholerae with an immunochromatographic test strip that can detect as low as 10 ng/mL of purified recombinant CT.
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