-transparent.png){kind=logo}
A quantitative gas chromatographic mass spectrometric method using a deuterated analogue as internal standard were developed for the analysis of 5-methoxytryptamine in brain. The internal standard was synthesized in two steps and possessed high isotopic purity. The analytical procedure involves the addition of the internal standard to the homogenizing medium containing the tissue, followed by homogenization, extraction with chloroform and derivatization with pentafluoropropionic anhydride. The identification and quantitation of 5-methoxytryptamine is based on monitoring the ions at m/z 306, 319 and 482 for the pentafluoropropionyl derivative of 5-methoxytryptamine and m/z 308, 322 and 486 for the internal standard. Using this procedure 5-methoxytryptamine was identified and quantitated in the sheep pineal gland. The mean 5-methoxytryptamine level (N = 12) was 545 +/- 180 pmol g-1.
O Beck, and
T R Bosin
November 2006,
Journal of chromatography. A,
O Beck, and
T R Bosin
July 1996,
Journal of chromatography. B, Biomedical applications,
O Beck, and
T R Bosin
January 2021,
Methods in molecular biology (Clifton, N.J.),
O Beck, and
T R Bosin
January 1990,
Methods in enzymology,
O Beck, and
T R Bosin
January 1973,
Advances in biochemical psychopharmacology,
O Beck, and
T R Bosin
September 2015,
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology,
O Beck, and
T R Bosin
November 1994,
American journal of clinical pathology,
O Beck, and
T R Bosin
January 1979,
Birth defects original article series,
O Beck, and
T R Bosin
October 1999,
Journal of mass spectrometry : JMS,
O Beck, and
T R Bosin
January 1974,
Life sciences,
