Effects of Bothrops alternatus venom in zebrafish: a histopathological study. 2018

José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Macapá, AP, 68902-280, Brazil. farmacos@unifap.br.

Zebrafish is an excellent model organism for studying tissue alterations caused by Bothrops alternatus venom (BAV) and for screening new anti-venom drugs. To study tissue alterations following exposure to BAV and the roles that glucocorticoids play in these tissue reactions, zebrafish were randomly divided into five groups: the free injection control group (FIC), the phosphate-buffered saline injection control group (PIC), the venom injected group (VI), the group treated with dexamethasone 1 h before venom injection (D1hBVI) and the group treated with dexamethasone 1 h after venom injection (D1hAVI). The concentration of BAV injected was 0.13 mg/mL and each fish received an injection of 20 μL. Body weight measurements and histopathological characteristics of the gills, kidneys, liver, and intestine were determined. Histopathological analyses showed necrosis, inflammation and weight gain in animals that received BAV. The histological alteration indices of the gills, liver, kidneys, and intestines were statistically higher in the animal groups treated with BAV. These alteration indices were lower in the D1hBVI and D1hAVI groups compared to the group treated with BAV alone. The D1hBVI group is presented with minor alterations. A significant difference in the histological alterations index was observed in the intestinal tissue of the FIC group compared to the PIC group. Cumulatively, zebrafish may serve as a useful biomarker for alterations induced by BAV. Interestingly, dexamethasone reduced the damage caused by BAV in the organs studied, which suggests that zebrafish might be useful for screening new drugs that can mitigate tissue damage caused by snakebites.

UI MeSH Term Description Entries
D007249 Inflammation A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function. Innate Inflammatory Response,Inflammations,Inflammatory Response, Innate,Innate Inflammatory Responses
D008297 Male Males
D001835 Body Weight The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms. Body Weights,Weight, Body,Weights, Body
D003435 Crotalid Venoms Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized. Bothrops Venom,Crotalidae Venoms,Pit Viper Venoms,Rattlesnake Venoms,Crotactin,Crotalid Venom,Crotalin,Crotaline Snake Venom,Crotalotoxin,Crotamin,Pit Viper Venom,Rattlesnake Venom,Snake Venom, Crotaline,Venom, Bothrops,Venom, Crotalid,Venom, Crotaline Snake,Venom, Pit Viper,Venom, Rattlesnake,Venoms, Crotalid,Venoms, Crotalidae,Venoms, Pit Viper,Venoms, Rattlesnake,Viper Venom, Pit
D003907 Dexamethasone An anti-inflammatory 9-fluoro-glucocorticoid. Hexadecadrol,Decaject,Decaject-L.A.,Decameth,Decaspray,Dexasone,Dexpak,Hexadrol,Maxidex,Methylfluorprednisolone,Millicorten,Oradexon,Decaject L.A.
D005938 Glucocorticoids A group of CORTICOSTEROIDS that affect carbohydrate metabolism (GLUCONEOGENESIS, liver glycogen deposition, elevation of BLOOD SUGAR), inhibit ADRENOCORTICOTROPIC HORMONE secretion, and possess pronounced anti-inflammatory activity. They also play a role in fat and protein metabolism, maintenance of arterial blood pressure, alteration of the connective tissue response to injury, reduction in the number of circulating lymphocytes, and functioning of the central nervous system. Glucocorticoid,Glucocorticoid Effect,Glucorticoid Effects,Effect, Glucocorticoid,Effects, Glucorticoid
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015027 Zebrafish An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. Zebrafish is a model organism for drug assay and cancer research. Brachydanio rerio,Danio rerio,B. rerio,D. rerio,Zebra Fish,Zebra Fishes,Zebra danio,Zebrafishes,D. rerios,Fishes, Zebra,Zebra danios,danio, Zebra
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D017837 Bothrops A genus of poisonous snakes of the VIPERIDAE family. About 50 species are known and all are found in tropical America and southern South America. Bothrops atrox is the fer-de-lance and B. jararaca is the jararaca. (Goin, Goin, and Zug, Introduction to Herpetology, 3d ed, p336)

Related Publications

José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
January 1984, Revista do Instituto de Medicina Tropical de Sao Paulo,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
February 2023, Toxicon : official journal of the International Society on Toxinology,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
December 1998, Toxicon : official journal of the International Society on Toxinology,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
December 2014, Comparative biochemistry and physiology. Part D, Genomics & proteomics,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
January 2010, Toxicon : official journal of the International Society on Toxinology,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
November 2002, Journal of protein chemistry,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
January 1996, Acta physiologica, pharmacologica et therapeutica latinoamericana : organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y [de] la Asociacion Latinoamericana de Farmacologia,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
April 2017, Apoptosis : an international journal on programmed cell death,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
November 2016, Toxicon : official journal of the International Society on Toxinology,
José Carlos Tavares Carvalho, and Hady Keita, and Giovanna Rocha Santana, and Gisele Custódio de Souza, and Igor Victor Ferreira Dos Santos, and Jesus Rafael Rodriguez Amado, and Ansoumane Kourouma, and Ariadna Lafourcade Prada, and Helison de Oliveira Carvalho, and Maria Lúcia Silva
September 2012, Cardiovascular toxicology,
Copied contents to your clipboard!