Bovine vaccinia: Inactivated Vaccinia virus vaccine induces protection in murine model. 2017

Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Brazil. Electronic address: matos.acd@gmail.com.

Bovine vaccinia (BV), caused by Vaccinia virus (VACV), is a zoonosis characterized by exanthematous lesions on the teats of dairy cows and the milkers' hands. Since 1999, due to the occurrence of many BV outbreaks in dairy farms across all Brazilian regions, there is a need to improve the control and prevention measures of the disease. Vaccination is one of the major tools to prevent viral diseases, and it could be an alternative for BV prevention. The main objective of this study was the development of vaccine formulations against BV using the inactivated VACV strain GP2 as antigen combined with different adjuvants. Potency tests were performed in mice, which were vaccinated with two doses at a 21-day interval, and then challenged with the vaccine homologous virus. VACV strain GP2 inactivated by beta-propiolactone (BPL) in association with adjuvants was effective in inducing a humoral immune response against VACV, as measured by neutralizing antibody (NA) titers, and was variable depending on the adjuvant used in each vaccine formulation. The vaccine formulation containing aluminum hydroxide (AH) associated with saponin as adjuvant induced the production of high NA titers in all vaccinated mice, giving 100% protection in Balb/c murine model after challenge with homologous virus.

UI MeSH Term Description Entries
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D010948 Viral Plaque Assay Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE. Bacteriophage Plaque Assay,Assay, Bacteriophage Plaque,Assay, Viral Plaque,Assays, Bacteriophage Plaque,Assays, Viral Plaque,Bacteriophage Plaque Assays,Plaque Assay, Bacteriophage,Plaque Assay, Viral,Plaque Assays, Bacteriophage,Plaque Assays, Viral,Viral Plaque Assays
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002418 Cattle Diseases Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus. Bovine Diseases,Bovine Disease,Cattle Disease,Disease, Bovine,Disease, Cattle,Diseases, Bovine,Diseases, Cattle
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D014615 Vaccinia The cutaneous and occasional systemic reactions associated with vaccination using smallpox (variola) vaccine. Vaccinias
D014616 Vaccinia virus The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS. Buffalopox virus,Poxvirus officinale,Rabbitpox virus,Buffalo Pox Virus,Rabbit Pox Virus,Buffalo Pox Viruses,Buffalopox viruses,Rabbit Pox Viruses,Rabbitpox viruses,Vaccinia viruses,Virus, Buffalo Pox,Viruses, Buffalo Pox,virus, Buffalopox
D014765 Viral Vaccines Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease. Viral Vaccine,Vaccine, Viral,Vaccines, Viral

Related Publications

Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
October 1971, The Journal of general virology,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
June 1988, Vaccine,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
January 2002, The new microbiologica,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
July 1995, The Veterinary record,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
January 1997, Avian diseases,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
October 1996, Veterinary microbiology,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
April 2001, Vaccine,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
June 2009, British journal of cancer,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
January 2009, The Journal of infectious diseases,
Ana Carolina Diniz Matos, and Maria Isabel Maldonado Coelho Guedes, and Izabelle Silva Rehfeld, and Erica Azevedo Costa, and Aristoteles Gomes Costa, and Natalia Lopes da Silva, and Andrey Pereira Lage, and Zélia Inês Portela Lobato
October 2017, Veterinary microbiology,
Copied contents to your clipboard!