-transparent.png){kind=logo}
The cell-free translation products of polyribosomal and post-polyribosomal mRNAs from the non-infective epimastigotes and the infective metacyclic trypomastigotes of the parasitic protozoan Trypanosoma cruzi were compared by two-dimensional polyacrylamide gel electrophoresis. The result show that although many polypeptides are conserved, quantitative and qualitative differences are observed between both differentiation stages. The results also indicate the existence of post-polyribosomal mRNAs in equilibrium with polyribosomal counterparts. The immunoprecipitation of the in vitro synthesized polypeptides with chagasic human serum and the serum raised against an 85-kDa glycoprotein (P2-WGA), potentially involved in the process of T. cruzi penetration into mammalian cells, shows that while the chagasic serum recognizes the same 72-kDa, 68-kDa and 46-kDa polypeptides in both differentiation stages, the anti-P2-WGA serum immunoprecipitates a single 48-kDa polypeptide from in vitro translation products of metacyclic trypomastigotes.
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
September 2021,
STAR protocols,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
December 2002,
Memorias do Instituto Oswaldo Cruz,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
July 1986,
Molecular and biochemical parasitology,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
July 1990,
Experimental parasitology,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
May 1983,
Infection and immunity,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
January 1986,
Revista do Instituto de Medicina Tropical de Sao Paulo,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
January 2022,
Current research in microbial sciences,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
December 1980,
The Journal of parasitology,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
March 1977,
Acta tropica,
S Goldenberg, and
J M Salles, and
V T Contreras, and
M P Lima Franco, and
A M Katzin, and
W Colli, and
C M Morel
June 1988,
Molecular and biochemical parasitology,
