BIOMAT Database Logo BIOMAT Database Logo

Antibodies under pressure: A Small-Angle X-ray Scattering study of Immunoglobulin G under high hydrostatic pressure. 2017

Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Fakultät Physik/DELTA, TU Dortmund, Dortmund 44221, Germany; Bayer AG, Leverkusen 51368, Germany. Electronic address: nico2.koenig@tu-dortmund.de.

In the present work two subclasses of the human antibody Immunoglobulin G (IgG) have been investigated by Small-Angle X-ray Scattering under high hydrostatic pressures up to 5kbar. It is shown that IgG adopts a symmetric T-shape in solution which differs significantly from available crystal structures. Moreover, high-pressure experiments verify the high stability of the IgG molecule. It is not unfolded by hydrostatic pressures of up to 5kbar but a slight increase of the radius of gyration was observed at elevated pressures.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006874 Hydrostatic Pressure The pressure due to the weight of fluid. Hydrostatic Pressures,Pressure, Hydrostatic,Pressures, Hydrostatic
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions
D017550 Spectroscopy, Fourier Transform Infrared A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained. FTIR,Fourier Transform Infrared Spectroscopy,Spectroscopy, Infrared, Fourier Transform
D053838 Scattering, Small Angle Scattering of a beam of electromagnetic or acoustic RADIATION, or particles, at small angles by particles or cavities whose dimensions are many times as large as the wavelength of the radiation or the de Broglie wavelength of the scattered particles. Also know as low angle scattering. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Small angle scattering (SAS) techniques, small angle neutron (SANS), X-ray (SAXS), and light (SALS, or just LS) scattering, are used to characterize objects on a nanoscale. Small Angle Scattering,Low Angle Scattering,Scattering, Low Angle,Angle Scattering, Low,Angle Scatterings, Low,Low Angle Scatterings,Scatterings, Low Angle

Related Publications

Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Inline small-angle X-ray scattering-coupled chromatography under extreme hydrostatic pressure.
December 2022, Protein science : a publication of the Protein Society,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
High Pressure Small-Angle X-Ray Scattering.
January 2015, Sub-cellular biochemistry,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Small angle x-ray scattering of a homogeneous gamm G-1 immunoglobulin.
January 1970, Biochemistry,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Structural characterization of lactate dehydrogenase dissociation under high pressure studied by synchrotron high-pressure small-angle X-ray scattering.
May 1999, Biochemistry,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Colloidal crystallite suspensions studied by high pressure small angle x-ray scattering.
February 2016, The Journal of chemical physics,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
X-ray small-angle scattering study of general structure of chicken immunoglobulin Y.
January 1982, Immunology letters,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Small-angle x-ray scattering.
December 1952, Nature,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Small-angle X-ray scattering.
January 1979, Methods in enzymology,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
Small-angle x-ray scattering.
January 1973, Methods in enzymology,
Nico König, and Michael Paulus, and Karin Julius, and Julian Schulze, and Matthias Voetz, and Metin Tolan
[Small-angle X-ray scattering method].
May 1994, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Copied contents to your clipboard!