The in vitro antimicrobial activity of different fractions obtained from rhizome of Curcuma longa was investigated against standard strain and clinical isolates of Staphylococcus aureus. The clinical isolates were found more sensitive for different fractions, than the standard strain of S. aureus. Scanning electron microscopic observations revealed that test pathogen treated with C. longa extract showed morphological deformity, with partial lack of the cytoplasmic membrane, which leads to cell disruption The ability of rhizome of C. longa extracts to inhibit the growth of test pathogen is an indication of its broad spectrum antimicrobial potential which may be employed in the management of microbial infections.