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In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer. Transformation was effected by using unfractionated high molecular weight genomic DNA from Chinese hamster, human, and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was between 1 and 10 colonies per 10(6) cells per 20 microgram of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into two classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
January 1985,
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
January 1995,
Neoplasma,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
December 1983,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
January 1983,
Progress in nucleic acid research and molecular biology,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
January 1983,
Molecular and cellular biology,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
March 1982,
Molecular and cellular biology,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
April 1991,
Current protocols in molecular biology,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
October 1998,
Science in China. Series C, Life sciences,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
March 1996,
Proceedings of the National Academy of Sciences of the United States of America,
M Wigler, and
A Pellicer, and
S Silverstein, and
R Axel, and
G Urlaub, and
L Chasin
January 1987,
Blood cells,
