BIOMAT Database Logo BIOMAT Database Logo

Comparison of rapid perfringens medium and lactose sulfite medium for detection of Clostridium perfringens. 1985

M Smith

Two tubed media, lactose sulfite (LS) and rapid perfringens medium (RPM), were evaluated and compared for their ability to detect and enumerate Clostridium perfringens in inoculated and naturally contaminated samples. In a 3-tube most probable number system, the number of organisms recovered from spiked samples was several-fold higher with RPM than with LS and was close to the numbers inoculated. Levels detected in 15 naturally contaminated samples were also higher with RPM. Only 13 of the samples were positive for C. perfringens with LS medium. These results indicate that RPM is a more effective medium than LS; therefore, its use is recommended.

UI MeSH Term Description Entries
D007785 Lactose A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry. Anhydrous Lactose,Lactose, Anhydrous
D003016 Clostridium perfringens The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins. Clostridium welchii
D003212 Condiments Aromatic substances added to food before or after cooking to enhance its flavor. These are usually of vegetable origin. Salad Dressing,Condiment,Dressing, Salad,Dressings, Salad,Salad Dressings
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food

Related Publications

M Smith
Rapid detection of Clostridium perfringens: comparison of lactose sulfite broth with tryptose-sulfite-cycloserine agar.
January 1985, Journal - Association of Official Analytical Chemists,
M Smith
Comparison of sulfite-polymyxin-sulfadiazine medium and tryptose-sulfite-cycloserine medium without egg yolk for recovering Clostridium perfringens.
April 1977, Applied and environmental microbiology,
M Smith
[Use of a selective sulfite-reducing medium for the isolation of Clostridium perfringens].
November 1979, Veterinarni medicina,
M Smith
[Sporulation medium for Clostridium perfringens].
January 1969, Annales de l'Institut Pasteur de Lille,
M Smith
New medium for rapid screening and enumeration of Clostridium perfringens in foods.
October 1978, Applied and environmental microbiology,
M Smith
A medium promoting rapid quantitative sporulation in Clostridium perfringens.
April 1956, Journal of bacteriology,
M Smith
Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.
September 1989, Applied and environmental microbiology,
M Smith
[Dry medium for isolating Clostridium perfringens].
April 1982, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
M Smith
Alternative medium for Clostridium perfringens sporulation.
May 1984, Applied and environmental microbiology,
M Smith
[Selective medium for Clostridium perfringens. II. Selective medium with sodium azide and crystal violet for isolation of Clostridium perfringens].
January 1973, Polskie archiwum weterynaryjne,
Copied contents to your clipboard!