microRNAs involved in auxin signalling modulate male sterility under high-temperature stress in cotton (Gossypium hirsutum). 2017

Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

Male sterility caused by long-term high-temperature (HT) stress occurs widely in crops. MicroRNAs (miRNAs), a class of endogenous non-coding small RNAs, play an important role in the plant response to various abiotic stresses. To dissect the working principle of miRNAs in male sterility under HT stress in cotton, a total of 112 known miRNAs, 270 novel miRNAs and 347 target genes were identified from anthers of HT-insensitive (84021) and HT-sensitive (H05) cotton cultivars under normal-temperature and HT conditions through small RNA and degradome sequencing. Quantitative reverse transcriptase-polymerase chain reaction and 5'-RNA ligase-mediated rapid amplification of cDNA ends experiments were used to validate the sequencing data. The results show that miR156 was suppressed by HT stress in both 84021 and H05; miR160 was suppressed in 84021 but induced in H05. Correspondingly, SPLs (target genes of miR156) were induced both in 84021 and H05; ARF10 and ARF17 (target genes of miR160) were induced in 84021 but suppressed in H05. Overexpressing miR160 increased cotton sensitivity to HT stress seen as anther indehiscence, associated with the suppression of ARF10 and ARF17 expression, thereby activating the auxin response that leads to anther indehiscence. Supporting this role for auxin, exogenous Indole-3-acetic acid (IAA) leads to a stronger male sterility phenotype both in 84021 and H05 under HT stress. Cotton plants overexpressing miR157 suppressed the auxin signal, and also showed enhanced sensitivity to HT stress, with microspore abortion and anther indehiscence. Thus, we propose that the auxin signal, mediated by miRNAs, is essential for cotton anther fertility under HT stress.

UI MeSH Term Description Entries
D007210 Indoleacetic Acids Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed) Auxin,Auxins,Indolylacetic Acids,Acids, Indoleacetic,Acids, Indolylacetic
D010937 Plant Growth Regulators Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins. Phytohormone,Phytohormones,Plant Growth Regulator,Plant Hormone,Plant Hormones,Growth Regulators, Plant,Regulators, Plant Growth,Growth Regulator, Plant,Hormone, Plant,Hormones, Plant,Regulator, Plant Growth
D003368 Gossypium A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES. Cotton Plant,Cotton Plants,Gossypiums,Plant, Cotton,Plants, Cotton
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D013312 Stress, Physiological The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions. Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D051479 Plant Infertility The failure of PLANTS to complete fertilization and obtain seed (SEEDS) as a result of defective POLLEN or ovules, or other aberrations. (Dict. of Plant Genet. and Mol. Biol., 1998) Infertility, Plant,Reproductive Sterility in Plants,Reproductive Sterility, Plants,Sterility, Reproductive, Plants
D035683 MicroRNAs Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing. RNA, Small Temporal,Small Temporal RNA,miRNA,stRNA,Micro RNA,MicroRNA,Primary MicroRNA,Primary miRNA,miRNAs,pre-miRNA,pri-miRNA,MicroRNA, Primary,RNA, Micro,Temporal RNA, Small,miRNA, Primary,pre miRNA,pri miRNA

Related Publications

Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
February 2020, International journal of molecular sciences,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
October 2019, Scientific data,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
April 2021, International journal of molecular sciences,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
June 2007, Journal of genetics and genomics = Yi chuan xue bao,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
January 2022, Frontiers in plant science,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
January 2006, Methods in molecular biology (Clifton, N.J.),
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
January 2015, Methods in molecular biology (Clifton, N.J.),
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
January 2024, Frontiers in plant science,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
November 2012, Science China. Life sciences,
Yuanhao Ding, and Yizan Ma, and Nian Liu, and Jiao Xu, and Qin Hu, and Yaoyao Li, and Yuanlong Wu, and Sai Xie, and Longfu Zhu, and Ling Min, and Xianlong Zhang
February 2021, TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik,
Copied contents to your clipboard!