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We describe a colorimetric method for assay of microsomal aminopeptidase (EC 3.4.11.2) activity in serum. We use a new substrate, L-leucyl-3-carboxy-4-hydroxyanilide, p-xylenol as coupler, and sodium metaperiodate as oxidizing reagent. The colored substance formed by the oxidative condensation between p-xylenol and 5-aminosalicylic acid absorbs maximally at 635 nm, and can be directly measured in serum. In a previous method for this enzyme, L-leucyl-beta-naphthylamide was used as substrate and beta-naphthylamine, a carcinogenic reagent used as a standard in making the assay, was unsuitable for routine use. We found a close correlation between results obtained with the new, safer method and with the previous method.
M Shimamoto, and
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T Nagasawa, and
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T Akatsuka
May 1986,
Clinical chemistry,
M Shimamoto, and
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T Akatsuka
July 1973,
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M Shimamoto, and
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T Akatsuka
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M Shimamoto, and
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M Shimamoto, and
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Klinische Wochenschrift,
