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gamma-glutamyltranspeptidase (gamma-GT) activity of the rat submandibular gland was studied after pilocarpine stimulation. After a single i.p. dose pilocarpine or physiologic saline the animals were sacrificed in intervals within 24 h p.i. The glands were homogenized and enzyme activity was calculated by measuring the release of p-nitroanilid per minute where rates of product formation were linear towards time and DNA-concentration. The specific activity of gamma-GT rapidly declined after pilocarpine injection until 3-6 h p.i. when a plateau stage was seen. During 6-24 h p.i. the gamma-GT activity slowly increased but never reached the original level. The controls receiving physiologic saline showed only a slight reduction of gamma-GT activity. However, in the control animals the initial level of enzyme activity was restored after 24 h. A depletion of cellular glutathione by pilocarpine is unlikely to explain the decrease of gamma-GT activity observed. The results indicate that pilocarpine affects the enzyme itself.
G Isacsson, and
G Andersson
January 1989,
Zeitschrift fur Naturforschung. C, Journal of biosciences,
G Isacsson, and
G Andersson
July 1987,
Research communications in chemical pathology and pharmacology,
G Isacsson, and
G Andersson
January 1983,
Hypertension (Dallas, Tex. : 1979),
G Isacsson, and
G Andersson
July 1984,
Experientia,
G Isacsson, and
G Andersson
June 1959,
The Journal of pharmacology and experimental therapeutics,
G Isacsson, and
G Andersson
May 1981,
Experientia,
G Isacsson, and
G Andersson
August 1986,
FEBS letters,
G Isacsson, and
G Andersson
January 2009,
The journal of medical investigation : JMI,
G Isacsson, and
G Andersson
January 1977,
Enzyme,
G Isacsson, and
G Andersson
August 1989,
Shigaku = Odontology; journal of Nihon Dental College,
