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Release of AMP and adenosine from rat heart mitochondria was studied. The rate of appearance of extramitochondrial adenosine was independent of the extramitochondrial phosphate concentration between 5 and 20 mM. In the absence of exogenous, respiratory substrates or in the presence of glutamate/malate plus rotenone, the rate of appearance of adenosine was relatively low when phosphate was not added. The appearance of extramitochondrial AMP + adenosine was found to be directly proportional to the extra-mitochondrial phosphate concentration. Zn2+ (10 mM) decreased the rate of adenosine appearance by 90% and increased the rate of AMP appearance 6-fold. The mitochondrial preparations dephosphorylated exogenous AMP; this activity was inhibited by 10 mM Zn2+. We conclude that the adenosine appearing in the extramitochondrial space was not due to a direct release from the matrix, but instead was due to adenine nucleotide release with subsequent conversion to adenosine in the extramitochondrial space.
G K Asimakis, and
D E Wilson, and
V R Conti
June 1983,
Biochemical and biophysical research communications,
G K Asimakis, and
D E Wilson, and
V R Conti
December 1979,
FEBS letters,
G K Asimakis, and
D E Wilson, and
V R Conti
November 1987,
Molecular and cellular biochemistry,
G K Asimakis, and
D E Wilson, and
V R Conti
March 1988,
Pharmacological research communications,
G K Asimakis, and
D E Wilson, and
V R Conti
December 1991,
The Journal of biological chemistry,
G K Asimakis, and
D E Wilson, and
V R Conti
August 1985,
The Biochemical journal,
G K Asimakis, and
D E Wilson, and
V R Conti
October 1986,
Biochimica et biophysica acta,
G K Asimakis, and
D E Wilson, and
V R Conti
September 1994,
European journal of pharmacology,
G K Asimakis, and
D E Wilson, and
V R Conti
April 1985,
FEBS letters,
G K Asimakis, and
D E Wilson, and
V R Conti
January 1984,
Advances in experimental medicine and biology,
