Professional values are essential to nursing practice because they guide standards for working, provide a structure for evaluating behavior, and influence decisions making. The purpose of this study is to explore the perception of Saudi female nursing students on professional values and to assess the correlation between their perception of professional values in relation to their year of academic studies. We used a cross-sectional descriptive study where a survey was administered to 150 Saudi female nurses living in Riyadh. Results show that Saudi female nurses have a high perception of professional values relating to confidentiality, privacy, moral and legal rights, health and safety, and the work environment. Whereas Saudi nursing students have a low perception for participating in professional nursing activities, utilizing research in practice, peer review, public policy, and engaging in on-going self-evaluation. There was positive correlation between different professional values and academic years. The highest correlations were for the items related to caring and trust more than activism because nursing students at higher academic levels viewed the relationship with patients as more important than advancing health care systems through public policy, research, and professional organizations. In conclusion, nursing program administrators should put emphasis on improving the development of professional values through a role modeling approach to promote activism and professional values through the arrangement of meetings, exchange forums, and conferences with other nurses, managers, policy makers, innovators, and researchers within the nursing field.