-transparent.png){kind=logo}
The distribution and properties of receptors for corticotropin-releasing factor (CRF) were analyzed in the brain of cynomolgus monkeys. Binding of [125I]tyrosine-labeled ovine CRF to frontal cortex and amygdala membrane-rich fractions was saturable, specific, and time- and temperature-dependent, reaching equilibrium in 30 min at 23 degrees C. Scatchard analysis of the binding data indicated one class of high-affinity sites with a Kd of 1 nM and a concentration of 125 fmol/mg (approximately equal to 30% of the receptor number in monkey anterior pituitary membranes). As in the rat pituitary and brain, CRF receptors in monkey cerebral cortex and amygdala were coupled to adenylate cyclase. Autoradiographic analysis of specific CRF binding in brain sections revealed that the receptors were widely distributed in the cerebral cortex and limbic system. Receptor density was highest in the pars tuberalis of the pituitary and throughout the cerebral cortex, specifically in the prefrontal, frontal, orbital, cingulate, insular, and temporal areas, and in the cerebellar cortex. A very high binding density was also present in the hippocampus, mainly in the dentate gyrus, and in the arcuate nucleus and nucleus tuberis lateralis. A high binding density was present in the amygdaloid complex and mamillary bodies, olfactory tubercle, and medial portion of the dorsomedial nucleus of the thalamus. A moderate binding density was found in the nucleus accumbens, claustrum, caudate-putamen, paraventricular and posterior lateral nuclei of the thalamus, inferior colliculus, and dorsal parabrachial nucleus. A low binding density was present in the superior colliculus, locus coeruleus, substantia gelatinosa, preoptic area, septal area, and bed nucleus of the stria terminalis. These data demonstrate that receptors for CRF are present within the primate brain at areas related to the central control of visceral function and behavior, suggesting that brain CRF may serve as a neurotransmitter in the coordination of endocrine and neural mechanisms involved in the response to stress.
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
January 1985,
Federation proceedings,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
January 1987,
Annals of the New York Academy of Sciences,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
January 1986,
Nature,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
January 1987,
Neuropeptides,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
August 2015,
Brain research,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
October 2010,
Neuropeptides,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
June 1999,
The Journal of comparative neurology,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
July 1988,
Brain research,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
March 1983,
Biochemical and biophysical research communications,
M A Millan, and
D M Jacobowitz, and
R L Hauger, and
K J Catt, and
G Aguilera
January 2012,
International journal of endocrinology,
