Amphotericin B induces apoptosis-like programmed cell death in Naegleria fowleri and Naegleria gruberi. 2017

Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, San Pedro Zacatenco, 07360, Mexico City, Mexico.

Naegleria fowleri and Naegleria gruberi belong to the free-living amoebae group. It is widely known that the non-pathogenic species N. gruberi is usually employed as a model to describe molecular pathways in this genus, mainly because its genome has been recently described. However, N. fowleri is an aetiological agent of primary amoebic meningoencephalitis, an acute and fatal disease. Currently, the most widely used drug for its treatment is amphotericin B (AmB). It was previously reported that AmB has an amoebicidal effect in both N. fowleri and N. gruberi trophozoites by inducing morphological changes that resemble programmed cell death (PCD). PCD is a mechanism that activates morphological, biochemical and genetic changes. However, PCD has not yet been characterized in the genus Naegleria. The aim of the present work was to evaluate the typical markers to describe PCD in both amoebae. These results showed that treated trophozoites displayed several parameters of apoptosis-like PCD in both species. We observed ultrastructural changes, an increase in reactive oxygen species, phosphatidylserine externalization and a decrease in intracellular potassium, while DNA degradation was evaluated using the TUNEL assay and agarose gels, and all of these parameters are related to PCD. Finally, we analysed the expression of apoptosis-related genes, such as sir2 and atg8, in N. gruberi. Taken together, our results showed that AmB induces the morphological, biochemical and genetic changes of apoptosis-like PCD in the genus Naegleria.

UI MeSH Term Description Entries
D009253 Naegleria A free-living soil amoeba pathogenic to humans and animals. It occurs also in water and sewage. The most commonly found species in man is NAEGLERIA FOWLERI which is the pathogen for primary amebic meningoencephalitis in primates. Naeglerias
D000666 Amphotericin B Macrolide antifungal antibiotic produced by Streptomyces nodosus obtained from soil of the Orinoco river region of Venezuela. Amphocil,Amphotericin,Amphotericin B Cholesterol Dispersion,Amphotericin B Colloidal Dispersion,Fungizone
D000981 Antiprotozoal Agents Substances that are destructive to protozoans. Schizonticides,Agents, Antiprotozoal
D015800 Protozoan Proteins Proteins found in any species of protozoan. Proteins, Protozoan
D016848 Naegleria fowleri A species of parasitic protozoa having both an ameboid and flagellate stage in its life cycle. Infection with this pathogen produces PRIMARY AMEBIC MENINGOENCEPHALITIS. Naegleria fowlerus,fowlerus, Naegleria
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen
D053058 Trophozoites Cells or feeding stage in the life cycle of sporozoan protozoa. In the malarial parasite, the trophozoite develops from the MEROZOITE and then splits into the SCHIZONT. Trophozoites that are left over from cell division can go on to form gametocytes. Trophozoite
D020808 Central Nervous System Protozoal Infections Infections of the brain, spinal cord, or meninges by single celled organisms of the former subkingdom known as protozoa. The central nervous system may be the primary or secondary site of protozoal infection. These diseases may occur as OPPORTUNISTIC INFECTIONS or arise in immunocompetent hosts. CNS Protozoal Infections,Cerebral Protozoal Infections,Meningoencephalitis, Protozoal,Protozoal Infections, Central Nervous System,Acanthamoeba Meningoencephalitis,Amebic Meningoencephalitis,Balamuthia mandrillaris CNS Infection,Balamuthia mandrillaris Meningoencephalitis,Infection, Central Nervous System, Protozoal,Infections, Protozoal, Central Nervous System,Naegleria fowleri Infection,Naegleria fowleri Meningoencephalitis,Primary Amebic Meningoencephalitis,Protozoal Infections, Cerebral,Protozoal Meningoencephalitis,Sappinia diploidea Meningoencephalitis,Acanthamoeba Meningoencephalitides,Amebic Meningoencephalitides,Amebic Meningoencephalitides, Primary,Amebic Meningoencephalitis, Primary,Balamuthia mandrillaris Meningoencephalitides,CNS Protozoal Infection,Cerebral Protozoal Infection,Infection, CNS Protozoal,Infection, Cerebral Protozoal,Infection, Naegleria fowleri,Infections, CNS Protozoal,Meningoencephalitides, Acanthamoeba,Meningoencephalitides, Amebic,Meningoencephalitides, Balamuthia mandrillaris,Meningoencephalitides, Naegleria fowleri,Meningoencephalitides, Primary Amebic,Meningoencephalitides, Protozoal,Meningoencephalitides, Sappinia diploidea,Meningoencephalitis, Acanthamoeba,Meningoencephalitis, Amebic,Meningoencephalitis, Balamuthia mandrillaris,Meningoencephalitis, Naegleria fowleri,Meningoencephalitis, Primary Amebic,Meningoencephalitis, Sappinia diploidea,Naegleria fowleri Infections,Naegleria fowleri Meningoencephalitides,Primary Amebic Meningoencephalitides,Protozoal Infection, CNS,Protozoal Infection, Cerebral,Protozoal Infections, CNS,Protozoal Meningoencephalitides,Sappinia diploidea Meningoencephalitides

Related Publications

Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
October 2023, Phytomedicine : international journal of phytotherapy and phytopharmacology,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
November 1975, Antimicrobial agents and chemotherapy,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
October 2021, Pharmaceuticals (Basel, Switzerland),
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
December 1982, The Journal of parasitology,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
June 2020, European journal of cell biology,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
January 2022, Frontiers in cell and developmental biology,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
April 1991, Journal of the Egyptian Society of Parasitology,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
January 1970, Antimicrobial agents and chemotherapy,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
April 1983, Journal of clinical microbiology,
Roberto Cárdenas-Zúñiga, and Angélica Silva-Olivares, and José D' Artagnan Villalba-Magdaleno, and Virginia Sánchez-Monroy, and Jesús Serrano-Luna, and Mineko Shibayama
January 1982, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Copied contents to your clipboard!