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Proteins extracted from suboesophageal ganglia of Squilla mantis, an arthropod shown to be sensitive in vivo to opiates and to contain native opioid like peptide(s), were fractionated by gel filtration into three pools according to their molecular weight: A (Mr greater than 65,000), B (10,000 less than Mr less than 65,000) and C (Mr less than 10,000). None of these pools showed any immunoreactivity when radioimmunoassayed using antisera raised against Met-enkephalin either before or after sequential trypsin/carboxypeptidase B proteolysis. Further purification of pool C by HPLC followed by RIA using antibodies directed to Met-O-enkephalin,Leu-enkephalin,Dynorphin 1-13 and human beta-endorphin, showed only a trace amount of Met-enkephalin cross-reactivity (about 10 fmoles/mg of protein extract). No detectable amount of Leu- or Met-enkephalin was found after HPLC fractionation of proteolyzed pool B. Radioreceptor assay of HPLC fractions derived from trypsin/carboxypeptidase B treated pools B and C showed major areas of activity common to both pools, but nevertheless with differing retention times compared to the standard opioid peptides used.
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
March 1971,
The Journal of cell biology,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
January 1980,
Cell and tissue research,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
January 1995,
The Journal of comparative neurology,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
January 1964,
Nature,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
December 1964,
The Journal of experimental biology,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
February 2008,
Environmental toxicology,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
July 1992,
Journal of comparative physiology. A, Sensory, neural, and behavioral physiology,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
January 1986,
Experimental biology,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
January 1992,
Peptides,
A Palmisano, and
G Marino, and
V Di Marzo, and
H R Morris, and
T A Howlett, and
S Tomlin
January 1984,
Developmental and comparative immunology,
