Biomaterial Database

Polyethylene glycol-modified hemin having peroxidase activity in organic solvents. 1986

K Takahashi, and A Matsushima, and Y Saito, and Y Inada

Hemin, having two carboxyl groups, was coupled with monomethoxypolyethylene glycol, PEG, through the ester bond formed with carbodiimide. The PEG-modified hemin was readily soluble not only in neutral aqueous solution but also in organic solvents. Its absorption spectrum in 1,1,1-trichloroethane showed a sharp Soret band at 398 nm. The modified hemin catalyzed the peroxidase-reaction in organic solvent and in aqueous solution using hydrogen peroxide or peroxidized linolenic acid as hydrogen acceptor and o-phenylene diamine as hydrogen donor. The activity of PEG-hemin in 1,1,1-trichloroethane was greater than that in an aqueous solution; k1 values in 1,1,1-trichloroethane were 2.3 X 10(3) M-1 sec-1 with hydrogen peroxide and 7.0 X 10(2) M-1 sec-1 with peroxidized linolenic acid, and the value in an aqueous solution was 3.0 X 10 M-1 sec-1 with hydrogen peroxide.

UI	MeSH Term	Description	Entries
D008042	Linolenic Acids	Eighteen-carbon essential fatty acids that contain three double bonds.
D010544	Peroxidases		Ovoperoxidase
D011092	Polyethylene Glycols	Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.	Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D006418	Heme	The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.	Ferroprotoporphyrin,Protoheme,Haem,Heme b,Protoheme IX
D006427	Hemin	Chloro(7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-N(21),N(22),N(23),N(24)) ferrate(2-) dihydrogen.	Ferriprotoporphyrin,Hematin,Alkaline Hematin D-575,Chlorohemin,Ferrihaem,Ferriheme Chloride,Ferriprotoporphyrin IX,Ferriprotoporphyrin IX Chloride,Panhematin,Protohemin,Protohemin IX,Alkaline Hematin D 575,Chloride, Ferriheme,Chloride, Ferriprotoporphyrin IX,Hematin D-575, Alkaline
D006861	Hydrogen Peroxide	A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.	Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities
D012997	Solvents	Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)	Solvent
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D014240	Trichloroethanes	Chlorinated ethanes which are used extensively as industrial solvents. They have been utilized in numerous home-use products including spot remover preparations and inhalant decongestant sprays. These compounds cause central nervous system and cardiovascular depression and are hepatotoxic. Include 1,1,1- and 1,1,2-isomers.