BIOMAT Database Logo BIOMAT Database Logo

Molecular genotyping of human T-cell antigen receptor variable gene segments. 1986

M A Robinson, and T J Kindt

The gene complex encoding the beta chain of the T-cell antigen receptor (Tcr) in man was previously reported to contain a restriction fragment length polymorphism (RFLP) involving a single Bgl II site adjacent to the second constant region gene. This RFLP allowed assignment of Tcr beta genotypes in certain human families. In the present study, two different RFLP in a V beta gene family were detected using the murine probe V8.1 in genomic DNA samples digested with the restriction endonucleases Hind III and Bam HI. Use of these RFLP to mark the V beta gene complex allowed complete haplotype assignment in four of seven families studied and provided support for linkage of the V gene complex to the constant region genes. Different combinations of the C and two V region markers can result in eight possible distinct haplotypes. The observation of all but one of the eight possible haplotypes in parents of the families studied suggests that recombination events occur between the C and V region and among members of the V region subfamily marked by the V8.1 probe. These markers can be used for mapping studies of the V beta gene complex in man and will allow an appraisal of possible associations between Tcr beta genes and disease susceptibility.

UI MeSH Term Description Entries
D010375 Pedigree The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition. Family Tree,Genealogical Tree,Genealogic Tree,Genetic Identity,Identity, Genetic,Family Trees,Genealogic Trees,Genealogical Trees,Genetic Identities,Identities, Genetic,Tree, Family,Tree, Genealogic,Tree, Genealogical,Trees, Family,Trees, Genealogic,Trees, Genealogical
D011948 Receptors, Antigen, T-Cell Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (CD3 COMPLEX). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains. Antigen Receptors, T-Cell,T-Cell Receptors,Receptors, T-Cell Antigen,T-Cell Antigen Receptor,T-Cell Receptor,Antigen Receptor, T-Cell,Antigen Receptors, T Cell,Receptor, T-Cell,Receptor, T-Cell Antigen,Receptors, T Cell Antigen,Receptors, T-Cell,T Cell Antigen Receptor,T Cell Receptor,T Cell Receptors,T-Cell Antigen Receptors
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D006239 Haplotypes The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX. Haplotype
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

M A Robinson, and T J Kindt
Diversity and organization of human T cell receptor delta variable gene segments.
January 1989, The Journal of experimental medicine,
M A Robinson, and T J Kindt
The human T cell antigen receptor is encoded by variable, diversity, and joining gene segments that rearrange to generate a complete V gene.
June 1984, Cell,
M A Robinson, and T J Kindt
Human T-cell receptor variable gene segment families.
January 1995, Immunogenetics,
M A Robinson, and T J Kindt
Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination.
January 2007, Proceedings of the National Academy of Sciences of the United States of America,
M A Robinson, and T J Kindt
Allelic polymorphism of human T-cell receptor V alpha gene segments.
January 1993, Immunogenetics,
M A Robinson, and T J Kindt
Molecular analysis of the human T-cell-antigen receptor.
February 1985, Biochemical Society transactions,
M A Robinson, and T J Kindt
Analysis of human T-cell antigen receptor variable beta gene usage following vaccination with recombinant HBsAg.
April 1998, Digestive diseases and sciences,
M A Robinson, and T J Kindt
Sequence and diversity of variable gene segments coding for rabbit T-cell receptor beta chains.
January 1994, Immunogenetics,
M A Robinson, and T J Kindt
Evolution of the variable gene segments and recombination signal sequences of the human T-cell receptor alpha/delta locus.
October 2004, Immunogenetics,
M A Robinson, and T J Kindt
[Study on molecular evolution for both variable segments of immunoglobulin heavy chain and T cell receptor].
January 1989, Yi chuan xue bao = Acta genetica Sinica,
Copied contents to your clipboard!