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Buffalo pox virus produced typical pock lesions on the skin of rabbits at the site of primary inoculation following an incubation period of 48-72 hr. Gross lesions in internal organs, characterized by focal or diffuse necrotic areas on lung, liver and spleen were seen from day 5 post-inoculation (p. i.). Isolated lesions of approximately 2 mm diameter appeared in skin, stomach, intestine and uterus from day 7 p. i. Histopathological changes, i. e. intra-alveolar and intra-bronchial haemorrhages were seen in lungs and severe fatty changes were found in the liver. Multinuclear cells were detected in liver during recovery. Virus particles were demonstrated by electron microscopy in skin, lung, liver and spleen lesions.
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Nihon Jinzo Gakkai shi,
